PMID- 11766992
OWN - NLM
STAT- MEDLINE
DCOM- 20020510
LR  - 20171116
IS  - 1023-3830 (Print)
IS  - 1023-3830 (Linking)
VI  - 50
IP  - 11
DP  - 2001 Nov
TI  - Fractalkine induces chemotaxis and actin polymerization in human dendritic cells.
PG  - 529-33
AB  - OBJECTIVE AND DESIGN: Dendritic cells (DCs) are considered as the principle 
      initiators of immune responses by virtue of their ability to migrate into target 
      sites, process antigens and activate naive T cells. Here, the chemotactic 
      activity and intracellular signaling of fractalkine was analyzed and compared to 
      well known chemotaxins. METHODS: The mRNA-expression of G protein-coupled CX3CR1 
      was analyzed by RT-PCR. Chemotaxis was measured in 48-well Boyden chambers and 
      actin polymerization by flow cytometry. RESULTS: The mRNA-expression of CX3CR1 in 
      immature and mature DCs was revealed. Fractalkine elicited actin polymerization 
      and chemotaxis in a dose-dependent manner in DCs independent of their state of 
      maturation. CONCLUSIONS: These results show that immature and mature DCs express 
      mRNA for the CX3CRI and that fractalkine induces chemotaxis and migration 
      associated actin polymerization in immature as well as in mature DCs, contrasting 
      with the action of other chemokines such as RANTES or MIP-3beta which act only on 
      distinct maturation states of DCs.
FAU - Dichmann, S
AU  - Dichmann S
AD  - Department of Dermatology, University of Freiburg, Germany.
FAU - Herouy, Y
AU  - Herouy Y
FAU - Purlis, D
AU  - Purlis D
FAU - Rheinen, H
AU  - Rheinen H
FAU - Gebicke-Harter, P
AU  - Gebicke-Harter P
FAU - Norgauer, J
AU  - Norgauer J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Actins)
RN  - 0 (CCL19 protein, human)
RN  - 0 (CX3C Chemokine Receptor 1)
RN  - 0 (CX3CL1 protein, human)
RN  - 0 (Chemokine CCL19)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CX3CL1)
RN  - 0 (Chemokines, CC)
RN  - 0 (Chemokines, CX3C)
RN  - 0 (Membrane Proteins)
RN  - 0 (Polymers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Receptors, HIV)
SB  - IM
MH  - Actins/*metabolism
MH  - CX3C Chemokine Receptor 1
MH  - Chemokine CCL19
MH  - Chemokine CCL5/pharmacology
MH  - Chemokine CX3CL1
MH  - Chemokines, CC/pharmacology
MH  - Chemokines, CX3C/*pharmacology
MH  - Chemotaxis/*drug effects
MH  - Dendritic Cells/*drug effects/physiology
MH  - Humans
MH  - Membrane Proteins/*pharmacology
MH  - Polymers/metabolism
MH  - RNA, Messenger/analysis
MH  - Receptors, Cytokine/genetics
MH  - Receptors, HIV/genetics
EDAT- 2002/01/05 10:00
MHDA- 2002/05/11 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/05/11 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.1007/PL00000230 [pii]
AID - 10.1007/PL00000230 [doi]
PST - ppublish
SO  - Inflamm Res. 2001 Nov;50(11):529-33. doi: 10.1007/PL00000230.