PMID- 11767054
OWN - NLM
STAT- MEDLINE
DCOM- 20020516
LR  - 20190605
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 63
IP  - 11
DP  - 2001 Nov
TI  - Analysis of open reading frame 5 in Japanese porcine reproductive and respiratory 
      syndrome virus isolates by restriction fragment length polymorphism.
PG  - 1203-7
AB  - For the epidemiological survey of porcine reproductive and respiratory syndrome 
      virus (PRRSV) isolated in Japan, the open reading frame (ORF) 5 gene of 37 field 
      isolates in Chiba prefecture from 1991 to 1999 were analyzed by restriction 
      fragment length polymorphism (RFLP). Reverse tanscription-polymerase chain 
      reaction (RT-PCR) amplifying the ORF 5 gene detected 35 field isolates except for 
      2 isolates. RFLP analysis with MluI, HincII, SacII, HaeIII and MspI demonstrated 
      that 35 field isolates were divided into 14 distinct codes and 34 isolates were 
      distinguished from VR2332-derived modified live PRRSV vaccine, indicating the 
      existence of genetic diversity in PRRSV field isolates in Japan. Only one strain 
      98-4A had an RFLP pattern identical to the vaccine strain. Nine out of the 35 
      field isolates (25.7%) had the RFLP code 1-3-2-1-1 and these patterns occurred 
      throughout the examination period. This suggests that these isolates are the 
      prevailing strain of PRRS in Chiba prefecture. RFLP with 5 enzymes demonstrated 
      that various strains existed in the same year, and that there were different 
      codes on the same farm according to the year. These observations indicate that 
      there is a genetic variation among field isolates in very limited regions and 
      some viruses possess the ability to cause their own genomic substitutions within 
      a herd in a short time.
FAU - Itou, T
AU  - Itou T
AD  - Department of Preventive Veterinary Medicine and Animal Health, Nihon University 
      School of Veterinary Medicine, Fujisawa, Kanagawa, Japan.
FAU - Tazoe, M
AU  - Tazoe M
FAU - Nakane, T
AU  - Nakane T
FAU - Miura, Y
AU  - Miura Y
FAU - Sakai, T
AU  - Sakai T
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - DNA, Viral/chemistry/genetics/isolation & purification
MH  - Japan/epidemiology
MH  - *Open Reading Frames
MH  - Polymorphism, Restriction Fragment Length
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/isolation & purification
MH  - RNA, Viral/chemistry/genetics/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
EDAT- 2002/01/05 10:00
MHDA- 2002/05/17 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/05/17 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.1292/jvms.63.1203 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2001 Nov;63(11):1203-7. doi: 10.1292/jvms.63.1203.