PMID- 11767276
OWN - NLM
STAT- MEDLINE
DCOM- 20020130
LR  - 20191105
IS  - 0909-8836 (Print)
IS  - 0909-8836 (Linking)
VI  - 109
IP  - 6
DP  - 2001 Dec
TI  - Actinobacillus actinomycetemcomitans-induced expression of IL-1alpha and IL-1beta 
      in human gingival epithelial cells: role in IL-8 expression.
PG  - 393-401
AB  - Gingival epithelial cells (GEC) are the first cells of the host that encounter 
      the periodontal pathogens. and therefore their role in the initiation of the 
      inflammatory response is critical. We aimed to: 1) characterize the expression of 
      interleukin (IL)- Ialpha and IL-Ibeta in human gingiva and cultured GEC: 2) 
      demonstrate the ability of A. actinomycetemcomitans extracts to upregulate 
      IL-1alpha, IL-1beta and IL-8 expression in GEC in vitro: and 3) characterize the 
      role of IL-1alpha and IL-1beta in the induction of IL-8 expression in GEC in 
      vitro. Ten gingival biopsies (5 inflamed and 5 controls) and cultured GEC were 
      examined for IL-1alpha and IL-Ibeta using immunohistochemical techniques. GEC 
      were also challenged with A. actinomycetemcomitans extracts or IL-1alpha, and 
      secretion of IL-1 and IL-8 was determined by ELISA. In vivo, IL-lalpha and 
      IL-1beta were localized in the gingival epithelium and the infiltrating 
      leukocytes. In vitro, A. actinomycetemcomitans extracts induced a time-dependent 
      expression of IL-1alpha, IL-1beta and IL-8 in GEC. IL-1 inhibitors did not affect 
      A. actinomycetemcomitans-induced IL-8. although they inhibited IL-8 induced by 
      IL-1alpha or IL-1beta. In conclusion, GEC are a major source of IL-1alpha and 
      IL-1beta in the periodontium, which in turn induce additional inflammatory 
      mediators such as IL-8. Therefore GEC can be a potential target for therapeutic 
      intervention in the future.
FAU - Sfakianakis, A
AU  - Sfakianakis A
AD  - Department of Periodontology, University of Connecticut, School of Medicine, 
      School of Dental Medicine, Farmington 06030-3105, USA.
FAU - Barr, C E
AU  - Barr CE
FAU - Kreutzer, D L
AU  - Kreutzer DL
LA  - eng
PT  - Journal Article
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Aggregatibacter actinomycetemcomitans/*physiology
MH  - Cell Line, Transformed
MH  - Epithelial Cells/metabolism/microbiology
MH  - Gingiva/cytology/*metabolism/*microbiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-1/*biosynthesis
MH  - Interleukin-8/*biosynthesis
MH  - Periodontitis/metabolism/microbiology
MH  - Up-Regulation
EDAT- 2002/01/05 10:00
MHDA- 2002/01/31 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/01/31 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.1034/j.1600-0722.2001.00162.x [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2001 Dec;109(6):393-401. doi: 10.1034/j.1600-0722.2001.00162.x.