PMID- 11768134
OWN - NLM
STAT- MEDLINE
DCOM- 20020423
LR  - 20181113
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 65
IP  - 4
DP  - 2001 Oct
TI  - Transmission of porcine reproductive and respiratory syndrome virus from 
      persistently infected sows to contact controls.
PG  - 261-6
AB  - The objective of this study was to determine if porcine reproductive and 
      respiratory syndrome virus (PRRSV) could persist in non-pregnant sows and if 
      persistently infected sows could transmit virus to naive contact controls. Twelve 
      PRRSV-naive, non-pregnant sows (index sows) were infected with a field isolate of 
      PRRSV and housed in individual isolation rooms for 42 to 56 days postinfection. 
      Following this period, 1 naive contact sow was placed in each room divided by a 
      gate allowing nose-to-nose contact with a single index sow. Index sows were not 
      viremic at the time of contact sow entry. Virus nucleic acid was detected by 
      polymerase chain reaction, and infectious virus was detected by virus isolation 
      in sera from 3 of the 12 contact sows at 49, 56, and 86 days postinfection. All 3 
      infected contacts developed PRRSV antibodies. Virus nucleic acid was detected in 
      tissues of all of the 12 index sows at 72 or 86 days postinfection. Nucleic acid 
      sequencing indicated that representative samples from index and infected contacts 
      were homologous (> 99%) to the PRRSV used to infect index sows at the onset of 
      the study. This study demonstrates that PRRSV can persist in sows and that 
      persistently infected sows can transmit virus to naive contact animals.
FAU - Bierk, M D
AU  - Bierk MD
AD  - Center for Swine Disease Eradication, Department of Clinical and Population 
      Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul 
      55108, USA.
FAU - Dee, S A
AU  - Dee SA
FAU - Rossow, K D
AU  - Rossow KD
FAU - Otake, S
AU  - Otake S
FAU - Collins, J E
AU  - Collins JE
FAU - Molitor, T W
AU  - Molitor TW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Base Sequence
MH  - Chronic Disease
MH  - Disease Transmission, Infectious/*veterinary
MH  - Female
MH  - Polymerase Chain Reaction/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/blood/pathology/*transmission
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/immunology/*isolation & purification
MH  - RNA, Viral/blood
MH  - Sequence Homology
MH  - Swine
MH  - Viremia/diagnosis/virology
MH  - Virus Shedding
PMC - PMC1189689
EDAT- 2002/01/05 10:00
MHDA- 2002/04/24 10:01
PMCR- 2002/01/01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/04/24 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PHST- 2002/01/01 00:00 [pmc-release]
PST - ppublish
SO  - Can J Vet Res. 2001 Oct;65(4):261-6.