PMID- 11769614
OWN - NLM
STAT- MEDLINE
DCOM- 20020228
LR  - 20121115
IS  - 0529-5815 (Print)
IS  - 0529-5815 (Linking)
VI  - 39
IP  - 9
DP  - 2001 Sep
TI  - [Intraspinal implantation of genetically modified myoblasts with brain-derived 
      neurotrophic factor gene in treating spinal cord injury in rats: 
      neurophysiological study].
PG  - 724-7
AB  - OBJECTIVE: To observe the value of gene therapy to spinal cord injury in vivo by 
      transferring gene of brain-derived neurotrophic factor (BDNF) to rat myoblast 
      with retroviral vector electrophysiologically. METHODS: Transection of spinal 
      cord at the level of T9 was produced in 30 adult SD rats that were divided 
      randomly into three groups: BDNF gene modified myoblast implantation (group A), 
      myoblast implantation (group B), and no cell implantation as controls (group C). 
      Three months after operation, whether the axon had regenerated and how the neural 
      function was restored were observed, using the techniques of cerebral 
      somatosensory evoked potential (CSEP) and motor evoked potential (MEP). RESULTS: 
      Three months after injury, 3 rats in group A showed CSEP signals and 5 rats MEP 
      signals, but no electrophysiological response was found in group B or group C. 
      The amplitudes of the recovery of CSEP or MEP signals decreased and their 
      latencies increased as compared with those before injury. The neural function of 
      the spinal cord was greater in group A than in B and C. CONCLUSION: Treatment 
      with genetically modified myoblasts producing BDNF can accelerate recovery from 
      traumatic spinal cord injury in adult rats.
FAU - Yu, K
AU  - Yu K
AD  - Department of Orthopedics, Changzheng Hospital, Second Military Medical 
      University, Shanghai 201103, China.
FAU - Ye, X
AU  - Ye X
FAU - Qu, S
AU  - Qu S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Wai Ke Za Zhi
JT  - Zhonghua wai ke za zhi [Chinese journal of surgery]
JID - 0153611
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - *Cell Transplantation
MH  - Evoked Potentials, Motor
MH  - Evoked Potentials, Somatosensory
MH  - Female
MH  - Genetic Engineering
MH  - *Genetic Therapy
MH  - Male
MH  - Muscles/*cytology/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord Injuries/physiopathology/*therapy
EDAT- 2002/01/05 10:00
MHDA- 2002/03/01 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/03/01 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Wai Ke Za Zhi. 2001 Sep;39(9):724-7.