PMID- 11769675
OWN - NLM
STAT- MEDLINE
DCOM- 20020115
LR  - 20061115
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 22
IP  - 10
DP  - 2001 Oct
TI  - [Detection of -5/5q- chromosome abnormality in myelodysplastic syndromes by 
      interphase fluorescence in situ hybridization].
PG  - 517-9
AB  - OBJECTIVE: To evaluate the value of interphase fluorescence in situ 
      hybridization(FISH) in the detection of acquired complete and partial deletion of 
      chromosome 5(-5/5q-) in myelodysplastic syndromes (MDS). METHODS: Marrow cells 
      from 48 MDS patients and 10 normal controls were studied by interphase FISH using 
      SpectrumRed directly labelled DNA specific probe for 5q31, 200 to 300 cells were 
      scored for each case and cells with a red hybridization signal > 6% were defined 
      as -5/5q- positive. RESULTS: In 48 MDS patients, 13 were positive for interphase 
      FISH, of whom, 7 were positive and 6 were negative for conventional cytogenetics 
      (CC). The difference in the percentage of positive cells detected by the two 
      methods was statistically significant (P < 0.05). CONCLUSION: Interphase FISH is 
      more sensitive than CC for the detection of -5/5q- in MDS.
FAU - Shen, Y
AU  - Shen Y
AD  - First Affiliated Hospital, Soochow University, Jiangsu Institute of Hematology, 
      Suzhou 215006, China.
FAU - Xue, Y
AU  - Xue Y
FAU - Li, J
AU  - Li J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 5/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics
EDAT- 2002/01/05 10:00
MHDA- 2002/01/16 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/01/16 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2001 Oct;22(10):517-9.