PMID- 11770903
OWN - NLM
STAT- MEDLINE
DCOM- 20020530
LR  - 20210904
IS  - 1015-6305 (Print)
IS  - 1750-3639 (Electronic)
IS  - 1015-6305 (Linking)
VI  - 12
IP  - 1
DP  - 2002 Jan
TI  - Fluorescence in situ hybridization (FISH) in diagnostic and investigative 
      neuropathology.
PG  - 67-86
AB  - Over the last decade, fluorescence in situ hybridization (FISH) has emerged as a 
      powerful clinical and research tool for the assessment of target DNA dosages 
      within interphase nuclei. Detectable alterations include aneusomies, deletions, 
      gene amplifications, and translocations, with primary advantages to the 
      pathologist including its basis in morphology, its applicability to archival, 
      formalin-fixed paraffin-embedded (FFPE) material, and its similarities to 
      immunohistochemistry. Recent technical advances such as improved hybridization 
      protocols, markedly expanded probe availability resulting from the human genome 
      sequencing initiative, and the advent of high-throughput assays such as gene chip 
      and tissue microarrays have greatly enhanced the applicability of FISH. In our 
      lab, we currently utilize only a limited battery of DNA probes for routine 
      diagnostic purposes, with determination of chromosome 1p and 19q dosage in 
      oligodendroglial neoplasms representing the most common application. However, 
      research applications are numerous and will likely translate into a growing list 
      of clinically useful markers in the near future. In this review, we highlight the 
      advantages and disadvantages of FISH and familiarize the reader with current 
      applications in diagnostic and investigative neuropathology.
FAU - Fuller, Christine E
AU  - Fuller CE
AD  - Division of Neuropathology, Washington University School of Medicine, St. Louis, 
      MO 63110-1093, USA.
FAU - Perry, Arie
AU  - Perry A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Review
PL  - Switzerland
TA  - Brain Pathol
JT  - Brain pathology (Zurich, Switzerland)
JID - 9216781
RN  - 0 (Molecular Probes)
SB  - IM
MH  - Artifacts
MH  - Brain Neoplasms/*genetics/*pathology
MH  - *Chromosome Aberrations
MH  - DNA Mutational Analysis/*methods
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Molecular Probes
MH  - Reproducibility of Results
PMC - PMC8095867
EDAT- 2002/01/05 10:00
MHDA- 2002/05/31 10:01
PMCR- 2006/04/05
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/05/31 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PHST- 2006/04/05 00:00 [pmc-release]
AID - BPA67 [pii]
AID - 10.1111/j.1750-3639.2002.tb00424.x [doi]
PST - ppublish
SO  - Brain Pathol. 2002 Jan;12(1):67-86. doi: 10.1111/j.1750-3639.2002.tb00424.x.