PMID- 11771661
OWN - NLM
STAT- MEDLINE
DCOM- 20020617
LR  - 20071114
IS  - 0884-0431 (Print)
IS  - 0884-0431 (Linking)
VI  - 17
IP  - 1
DP  - 2002 Jan
TI  - Sequence analysis of measles virus nucleocapsid transcripts in patients with 
      Paget's disease.
PG  - 145-51
AB  - It has been debated for almost 30 years whether Paget's disease of bone results 
      from paramyxoviral infection of osteoclasts (OCs). Paramyxoviral-like nuclear 
      inclusions are found in OCs from patients with Paget's disease, and measles virus 
      (MV) or canine distemper virus (CDV) messenger RNA (mRNA) transcripts have been 
      detected by in situ hybridization in bone cells from pagetic lesions. 
      Furthermore, immunocytochemical studies have shown the presence of several 
      paramyxoviral species in OCs from patients with Paget's disease. However, others 
      have been unable to detect paramyxoviral transcripts in bone samples from 
      patients with Paget's disease or marrow cultures from involved sites of patients 
      with Paget's disease. Furthermore, no one has been able to isolate an infectious 
      virus from pagetic bone samples or marrow cells from patients with Paget's 
      disease, and a full-length viral gene has not been sequenced from pagetic 
      samples. In this study, we have obtained the full-length sequence for the MV 
      nucleocapsid (MVNP) gene in bone marrow from an involved site from a patient with 
      Paget's disease and more than 700 base pairs (bps) of MVNP sequence in 3 other 
      patients with Paget's disease. These sequences were undetectable in four normal 
      marrow samples studied simultaneously. The sequences from the patients contained 
      multiple mutations that differed from the Edmonston strain MVNP gene. These 
      findings are consistent with the presence of a chronic MV infection in affected 
      sites from these patients with Paget's disease.
FAU - Friedrichs, William E
AU  - Friedrichs WE
AD  - Department of Medicine/Hematology, University of Texas Health Science Center, San 
      Antonio, USA.
FAU - Reddy, Sakamuri V
AU  - Reddy SV
FAU - Bruder, Jan M
AU  - Bruder JM
FAU - Cundy, Tim
AU  - Cundy T
FAU - Cornish, Jillian
AU  - Cornish J
FAU - Singer, Frederick R
AU  - Singer FR
FAU - Roodman, G David
AU  - Roodman GD
LA  - eng
GR  - AG13625/AG/NIA NIH HHS/United States
GR  - AR44603/AR/NIAMS NIH HHS/United States
GR  - DE12603/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American 
      Society for Bone and Mineral Research
JID - 8610640
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
CIN - J Bone Miner Res. 2002 Dec;17(12):2290-2; author reply 2293. PMID: 12469924
MH  - Aged
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Case-Control Studies
MH  - Humans
MH  - Measles virus/*genetics/*isolation & purification/pathogenicity
MH  - Molecular Sequence Data
MH  - Nucleocapsid Proteins/*genetics
MH  - Osteitis Deformans/etiology/*virology
MH  - Osteoclasts/virology
MH  - Polymorphism, Genetic
MH  - RNA, Messenger/*genetics/*isolation & purification
MH  - RNA, Viral/*genetics/*isolation & purification
MH  - Sequence Analysis, RNA
MH  - Sequence Homology, Amino Acid
EDAT- 2002/01/05 10:00
MHDA- 2002/06/18 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/06/18 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.1359/jbmr.2002.17.1.145 [doi]
PST - ppublish
SO  - J Bone Miner Res. 2002 Jan;17(1):145-51. doi: 10.1359/jbmr.2002.17.1.145.