PMID- 11773123
OWN - NLM
STAT- MEDLINE
DCOM- 20020212
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 40
IP  - 1
DP  - 2002 Jan
TI  - Rapid identification of Staphylococcus aureus directly from blood cultures by 
      fluorescence in situ hybridization with peptide nucleic acid probes.
PG  - 247-51
AB  - A new fluorescence in situ hybridization (FISH) method with peptide nucleic acid 
      (PNA) probes for identification of Staphylococcus aureus directly from positive 
      blood culture bottles that contain gram-positive cocci in clusters (GPCC) is 
      described. The test (the S. aureus PNA FISH assay) is based on a 
      fluorescein-labeled PNA probe that targets a species-specific sequence of the 16S 
      rRNA of S. aureus. Evaluations with 17 reference strains and 48 clinical 
      isolates, including methicillin-resistant and methicillin-susceptible S. aureus 
      species, coagulase-negative Staphylococcus species, and other clinically relevant 
      and phylogenetically related bacteria and yeast species, showed that the assay 
      had 100% sensitivity and 96% specificity. Clinical trials with 87 blood cultures 
      positive for GPCC correctly identified 36 of 37 (97%) of the S. aureus-positive 
      cultures identified by standard microbiological methods. The positive and 
      negative predictive values were 100 and 98%, respectively. It is concluded that 
      this rapid method (2.5 h) for identification of S. aureus directly from blood 
      culture bottles that contain GPCC offers important information for optimal 
      antibiotic therapy.
FAU - Oliveira, Kenneth
AU  - Oliveira K
AD  - Boston Probes, Inc., Bedford, Massachusetts 01730, USA.
FAU - Procop, Gary W
AU  - Procop GW
FAU - Wilson, Deborah
AU  - Wilson D
FAU - Coull, James
AU  - Coull J
FAU - Stender, Henrik
AU  - Stender H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Culture Media)
RN  - 0 (Nucleic Acid Probes)
RN  - 0 (Peptide Nucleic Acids)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Blood/*microbiology
MH  - Culture Media
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Nucleic Acid Probes
MH  - Peptide Nucleic Acids/*genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sensitivity and Specificity
MH  - Staphylococcal Infections/microbiology
MH  - Staphylococcus aureus/*classification/genetics/isolation & purification
MH  - Time Factors
PMC - PMC120122
EDAT- 2002/01/05 10:00
MHDA- 2002/02/13 10:01
PMCR- 2002/01/01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/02/13 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PHST- 2002/01/01 00:00 [pmc-release]
AID - 0890 [pii]
AID - 10.1128/JCM.40.1.247-251.2002 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2002 Jan;40(1):247-51. doi: 10.1128/JCM.40.1.247-251.2002.