PMID- 11773426
OWN - NLM
STAT- MEDLINE
DCOM- 20020212
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 76
IP  - 3
DP  - 2002 Feb
TI  - Kissing interaction between 3' noncoding and coding sequences is essential for 
      porcine arterivirus RNA replication.
PG  - 1521-6
AB  - We used an infectious cDNA clone of porcine reproductive and respiratory syndrome 
      virus (PRRSV) to investigate the presence of essential replication elements in 
      the region of the genome encoding the structural proteins. Deletion analysis 
      showed that a stretch of 34 nucleotides (14653 to 14686) within ORF7, which 
      encodes the nucleocapsid protein, is essential for RNA replication. 
      Strand-specific reverse transcription-PCR analysis of viral RNA isolated from 
      transfected BHK-21 cells revealed that this region is required for 
      negative-strand genomic RNA synthesis. The 34-nucleotide stretch is highly 
      conserved among PRRSV isolates and folds into a putative hairpin. A 7-base 
      sequence within the loop of this structure was suggested to base-pair with a 
      sequence present in the loop of a hairpin located in the 3' noncoding region, 
      resulting in a kissing interaction. Mutational analyses confirmed that this 
      kissing interaction is required for RNA replication.
FAU - Verheije, M H
AU  - Verheije MH
AD  - Department of Infectious Diseases and Food Chain Quality, Institute for Animal 
      Science and Health, Lelystad, The Netherlands. h.verheije@vet.uu.nl
FAU - Olsthoorn, R C L
AU  - Olsthoorn RC
FAU - Kroese, M V
AU  - Kroese MV
FAU - Rottier, P J M
AU  - Rottier PJ
FAU - Meulenberg, J J M
AU  - Meulenberg JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (3' Untranslated Regions)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - 3' Untranslated Regions/*physiology
MH  - Animals
MH  - Cell Line
MH  - Cricetinae
MH  - Nucleic Acid Conformation
MH  - Nucleocapsid/genetics
MH  - Open Reading Frames
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/physiology
MH  - RNA, Viral/*physiology
MH  - Swine
MH  - Viral Matrix Proteins/genetics
MH  - Viral Nonstructural Proteins/genetics
MH  - Virus Replication/*genetics
PMC - PMC135790
EDAT- 2002/01/05 10:00
MHDA- 2002/02/13 10:01
PMCR- 2002/02/01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/02/13 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PHST- 2002/02/01 00:00 [pmc-release]
AID - 1142 [pii]
AID - 10.1128/jvi.76.3.1521-1526.2002 [doi]
PST - ppublish
SO  - J Virol. 2002 Feb;76(3):1521-6. doi: 10.1128/jvi.76.3.1521-1526.2002.