PMID- 11774208
OWN - NLM
STAT- MEDLINE
DCOM- 20020207
LR  - 20111117
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 18
IP  - 6
DP  - 2001 Dec
TI  - [The impact of calpain-10 gene combined-SNP variation on type 2 diabetes mellitus 
      and its related metabolic traits].
PG  - 426-30
AB  - OBJECTIVE: To investigate the impact of calpain-10 gene (CAPN-10) combined single 
      nucleotide polymorphism (SNP) variation on type 2 diabetes mellitus (T2DM) and 
      its related clinical metabolic traits in Chinese. METHODS: The study population 
      consisted of 268 Chinese residents in Shanghai. Among them, 144 were subjects 
      with normal glucose tolerance (NGT) and 124, with T2DM. Plasma glucose (PG), 
      insulin (INS), c-peptide (CP) and free fatty acids (FFA) levels were measured at 
      fasting and 30, 60, 120, and 180 minutes after oral 75 g glucose challenge. The 
      islet beta-cell insulin secretion and tissue insulin sensitivity were assessed. 
      CAPN-10 UCSNP44,-43,-19 and -63 were genotyped. RESULTS: (1) In Chinese NGT 
      subjects, the major allele of UCSNP-44 was allele T (frequency=91%), of UCSNP43 
      was G(89%), of UCSNP-19 was I (3 repeats of a 32 bp sequence) (67%) and of 
      UCSNP-63 was C allele (79%). Significant differences were observed in comparison 
      of these allele frequencies in Chinese to those in other ethnic groups reported 
      in the literature. (2) 14 genotype combinations of these four SNPs were observed 
      in Chinese NGT subjects. 69% of the NGT population was composed of four genotype 
      combinations, in the order of UCSNP44,-43,-19 and -63, i.e., combination 
      A:TT-GG-DI-CC(haplotype combination was 1121/1111) (frequency=10%), combination 
      B:TT-GA-II-CC(1121/1221)(10%), combination C:TT-GG-II-CC(1121/1121)(26%) and 
      combination D:TT-GG-DI-CT(1121/1112)(22%).(3) The frequencies of the above 
      mentioned SNP in single or in combinations were not different significantly 
      between NGT and T2DM groups. (4) The variation of clinical metabolic parameter 
      levels shifted from completely normal towards abnormal glucose intolerance among 
      genotype combination subgroups. In comparison between combination A and 
      combination D, subjects in the former subgroups had: higher PG levels with 
      delayed peak after glucose challenge; less and lower decrement of FFA levels 
      after challenge with no rising in late stage; higher insulin levels with delayed 
      peak after challenge; and the tendency of decreased insulin sensitivity. More 
      than half of the comparisons remained statistically significant after adjusted 
      with age, gender, body mass index and waist circumference. CONCLUSION: The 
      variation of calpain-10 gene has impact on the variation of clinical metabolic 
      parameter levels related to type 2 diabetes mellitus. Such impact depends upon 
      the haplotypes as well as the haplotype combination of calpain-10 gene 
      variations.
FAU - Xiang, K
AU  - Xiang K
AD  - Shanghai Diabetes Institute, Department of Endocrinology & Metabolism, Shanghai 
      No. 6 People Hospital, Shanghai, 200233 P.R.China. sphxiang@public.sta.net.cn
FAU - Fang, Q
AU  - Fang Q
FAU - Zheng, T
AU  - Zheng T
FAU - Jia, W
AU  - Jia W
FAU - Wang, Y
AU  - Wang Y
FAU - Zhang, R
AU  - Zhang R
FAU - Li, J
AU  - Li J
FAU - Shen, K
AU  - Shen K
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Blood Glucose)
RN  - 0 (C-Peptide)
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 0 (Insulin)
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.22.- (Calpain)
RN  - EC 3.4.22.- (calpain 10)
SB  - IM
MH  - Alleles
MH  - Blood Glucose/metabolism
MH  - C-Peptide/blood
MH  - Calpain/*genetics
MH  - DNA/genetics
MH  - Diabetes Mellitus, Type 2/blood/*genetics/pathology
MH  - Fatty Acids, Nonesterified/blood
MH  - Female
MH  - Gene Frequency
MH  - Genetic Variation
MH  - Glucose Tolerance Test
MH  - Humans
MH  - Insulin/blood
MH  - Male
MH  - Middle Aged
MH  - *Polymorphism, Single Nucleotide
EDAT- 2002/01/05 10:00
MHDA- 2002/02/08 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/02/08 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2001 Dec;18(6):426-30.