PMID- 11775065
OWN - NLM
STAT- MEDLINE
DCOM- 20020521
LR  - 20191025
IS  - 0272-4340 (Print)
IS  - 0272-4340 (Linking)
VI  - 21
IP  - 4
DP  - 2001 Aug
TI  - The lateral hypothalamus is involved in the pathway mediating the hypotensive 
      response to cingulate cortex-cholinergic stimulation.
PG  - 341-56
AB  - 1. The injection of acetylcholine (ACh) into the medial prefrontal cortex (MPFC) 
      caused marked hypotensive response in either unanesthetized or anesthetized rats. 
      2. The present experiment was designed to investigate anatomical connections of 
      the ACh injection site in the MPFC with putative autonomic-related brain nuclei, 
      as well as their possible involvement in the mediation of the hypotensive 
      response to ACh. 3. For the above purpose, the bidirectional neuronal tracer 
      biotinylated dextran amine (BDA) was injected into Cg1 and Cg3 areas, within the 
      MPFC of male Wistar rats. Five days later the animals were sacrificed and brain 
      slices were processed and analyzed to determine neuronal projections efferent 
      from as well afferent to the MPFC. 4. Neuronal staining was more prominent in 
      regions ipsilateral to the BDA injection site. Prominent efferent projections of 
      the MPFC were observed in the contralateral MPFC: ipsi- and contralateral 
      amygdala and hypothalamus; ipsilateral septal area, diagonal band, and zona 
      incerta. 5. Similar but not equal patterns of neuronal labeling were observed 
      when BDA injections were performed within the two adjacent MPFC areas. BDA 
      injections centered in the ACh injection site in the Cg3 area caused strong 
      labeling in the septal area and diagonal band as well as an overall hypothalamic 
      labeling. Within the hypothalamus an intense cortical projection was observed in 
      the lateral hypothalamus (LH). BDA injections into the Cg1 area caused a more 
      evident labeling of the amygdaloid complex. 6. Neuronal cell bodies were evident 
      throughout the MPFC as well as in the sensory-motor cortex when BDA was injected 
      into the LH, thus indicating a massive ipsilateral cortical projection from the 
      Cg3 to the LH. 7. Bilateral NMDA-induced lesions within the LH caused a 
      significant attenuation of the depressor responses to ACh injection in the MPFC, 
      whereas unilateral lesions were marginally effective. These results indicate the 
      involvement of the LH in the mediation of the hypotensive response to ACh 
      injection into the MPFC as well as the bilateral distribution of the hypotensive 
      pathway.
FAU - Pajolla, G P
AU  - Pajolla GP
AD  - Department of Pharmacology, School of Medicine of Ribeirao Preto, University of 
      Sao Paulo, Brazil.
FAU - Crippa, G E
AU  - Crippa GE
FAU - Correa, S A
AU  - Correa SA
FAU - Moreira, K B
AU  - Moreira KB
FAU - Tavares, R F
AU  - Tavares RF
FAU - Correa, F M
AU  - Correa FM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Mol Neurobiol
JT  - Cellular and molecular neurobiology
JID - 8200709
RN  - 0 (Cholinergic Agents)
RN  - 0 (Dextrans)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (biotinylated dextran amine)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - 6SO6U10H04 (Biotin)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Axonal Transport/drug effects/physiology
MH  - Biotin/administration & dosage/*analogs & derivatives
MH  - Cholinergic Agents/*pharmacology
MH  - Dextrans/administration & dosage
MH  - Fluorescent Dyes/administration & dosage
MH  - Gyrus Cinguli/drug effects/*physiology
MH  - Hypotension/chemically induced/*physiopathology
MH  - Hypothalamic Area, Lateral/drug effects/*physiology
MH  - Injections, Intraventricular
MH  - Male
MH  - N-Methylaspartate/toxicity
MH  - Neural Pathways/drug effects/physiology
MH  - Prefrontal Cortex/drug effects/*physiology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2002/01/05 10:00
MHDA- 2002/05/22 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/05/22 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.1023/a:1012650021137 [doi]
PST - ppublish
SO  - Cell Mol Neurobiol. 2001 Aug;21(4):341-56. doi: 10.1023/a:1012650021137.