PMID- 11775534
OWN - NLM
STAT- MEDLINE
DCOM- 20020130
LR  - 20041117
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 113
IP  - 2
DP  - 2000 Feb
TI  - Effect of 1,25(OH)2D3 on the growth and apoptosis of breast cancer cell line 
      MCF-7.
PG  - 124-8
AB  - OBJECTIVE: To study the effect of 1, 25-dihydroxyvitamin D3(1,25(OH)2D3) on the 
      growth and apoptosis of breast cancer cell line MCF-7. METHODS: Cell number was 
      determined using the MTT method. Flow cytometric analysis was performed on cell 
      cycles, and the percentage of apoptosis was counted. Apoptotic cells were 
      quantified by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL), and bcl-2 protein expression was estimated with Western 
      blotting. RESULTS: After incubation with 1,25(OH)2D3 10(-7) mol/L for 48 hours, 
      MCF-7 cells exhibited significant growth in a dose- and time-dependent manner. 
      Flow cytometric analysis indicated that cell numbers in G0/G1 increased along 
      with increasing apoptotic peak and percentage. With microscope and electron 
      microscope observation, characteristics of apoptosis such as typical apoptotic 
      bodies were commonly found. TUNEL also showed that 1,25(OH)2D3 10(-8) mol/L and 
      10(-7) mol/L groups had significantly high apoptosis percentage than control 
      group with dose-dependence on induction apoptosis. And Western blot showed that 
      1,25(OH)2D3 10(-8) mol/L could down-regulate bcl-2 protein and 10(-7) mol/L could 
      almost block bcl-2 protein expression. CONCLUSIONS: 1,25(OH)2D3 can inhibit cell 
      growth with G0/G1 arrest, enhance the proliferation inhibition action of 
      adriamycin, and induce apoptosis which may result from the down-regulation of the 
      anti-apoptotic bcl-2 protein.
FAU - Zhang, J
AU  - Zhang J
AD  - Department of General Surgery, First Affiliated Hospital, Chongqing University of 
      Medical Sciences, Chongqing 400016, China.
FAU - Yao, Z
AU  - Yao Z
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - EC 1.14.- (Steroid Hydroxylases)
RN  - EC 1.14.- (vitamin D 1-alpha hydroxylase)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Breast Neoplasms/*drug therapy/pathology/ultrastructure
MH  - Cell Cycle/drug effects
MH  - Cell Division/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Microscopy, Electron
MH  - Proto-Oncogene Proteins c-bcl-2/drug effects/metabolism
MH  - Steroid Hydroxylases/*pharmacology
MH  - Time Factors
MH  - Tumor Cells, Cultured/drug effects
EDAT- 2002/01/05 10:00
MHDA- 2002/01/31 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/01/31 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2000 Feb;113(2):124-8.