PMID- 11775837
OWN - NLM
STAT- MEDLINE
DCOM- 20020214
LR  - 20131121
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 113
IP  - 10
DP  - 2000 Oct
TI  - The effects of glucose, insulin and oxidized low density lipoprotein on apoptosis 
      in vascular endothelial cells.
PG  - 903-6
AB  - OBJECTIVE: To study the effects of high concentrations of glucose, insulin and 
      oxidized low density lipoprotein (ox-LDL) on apoptosis in cultured bovine aortic 
      endothelial cells (ECs). METHODS: For qualitative determination of EC apoptosis, 
      acridine orange (AO)/ethidium bromide (EB) staining and DNA agarose gels 
      electrophoresis were used. Cellular DNA fragmentation ELISA measured apoptosis by 
      quantitating the fragmentation of 5-bromo-2'-deoxyuridine-labeled DNA. RESULTS: 
      High concentrations of glucose (20 mmol/L, 40 mmol/L), insulin (3000 microU/ml) 
      and ox-LDL (50 micrograms/ml, 100 micrograms/ml) induced concentration- and 
      time-dependent apoptosis in ECs. They had a synergetic effect on EC apoptosis. 
      The combined effect of high concentration of glucose, insulin and ox-LDL was 
      greater than any two of them; the effect of two was greater than one alone. Low 
      concentration of insulin (30 microU/ml) decreased apoptosis in ECs induced by 
      high concentrations of glucose (40 mmol/L), but no similar effect occurred with 
      ox-LDL (100 micrograms/ml). CONCLUSION: High ambient glucose, insulin and ox-LDL 
      can induce excessive apoptosis in cultured ECs, and low ambient insulin can 
      prevent EC apoptosis. Excessive EC apoptosis induced by the separate or 
      synergetic effect of hyperglycemia, hyperinsulinemia and hyperlipidemia may be 
      one of the reasons for loss of endothelial integrity, dysfunction of the vascular 
      endothelium and increased plasma membrane permeability, which are all involved in 
      the development of diabetic macrovascular complications.
FAU - Chai, W
AU  - Chai W
AD  - Department of Endocrinology, First Affiliated Hospital, Nanjing Medical 
      University, Nanjing 210029, China.
FAU - Chen, J
AU  - Chen J
FAU - Wang, H
AU  - Wang H
FAU - Shen, J
AU  - Shen J
FAU - Ma, L
AU  - Ma L
FAU - Ma, X
AU  - Ma X
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Insulin)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (oxidized low density lipoprotein)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cattle
MH  - Cells, Cultured
MH  - DNA Fragmentation
MH  - Diabetic Angiopathies/etiology
MH  - Dose-Response Relationship, Drug
MH  - Endothelium, Vascular/*drug effects/pathology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glucose/*pharmacology
MH  - Insulin/*pharmacology
MH  - Lipoproteins, LDL/*pharmacology
EDAT- 2002/01/05 10:00
MHDA- 2002/02/15 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/02/15 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2000 Oct;113(10):903-6.