PMID- 11776150
OWN - NLM
STAT- MEDLINE
DCOM- 20020130
LR  - 20071119
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 113
IP  - 12
DP  - 2000 Dec
TI  - Human monocyte-derived dendritic cells expressing both chemotactic cytokines 
      IL-8, MCP-1, RANTES and their receptors, and their selective migration to these 
      chemokines.
PG  - 1124-8
AB  - OBJECTIVE: To characterize the mRNA expression of CXC chemokine IL-8, CC 
      chemokine monocyte chemothractant protein-1 (MCP-1) and regulated on activation, 
      normal T cell expressed and secreted (RANTES), and a newly defined DC chemokine 
      DC-CK1 as well as the expression of IL-8 receptor, MCP-1 receptor and RANTES 
      receptor in human monocyte-derived dendritic cells (MoDCs). The migratory 
      responsiveness of MoDC to IL-8, MCP-1 and RANTES was also studied. METHODS: In 
      vitro generated MoDCs were obtained by differentiating monocytes in the presence 
      of GM-CSF and IL-4 for 5 days. The time course of RNA expression was analyzed by 
      RT-PCR and migratory ability was assessed by a micromultiwell chemotaxis chamber 
      assay. RESULTS: IL-8, MCP-1, RANTES and their corresponding receptors were 
      consistently expressed in MoDCs. DC-CK-1 expression was detectable after 48 hours 
      of differentiation. MoDC selectively migrated in response to MCP-1 and RANTES but 
      not to IL-8 though transcripts of IL-8 receptor were present. CONCLUSION: Because 
      the capacity of dendritic cells to initiate immune responses depends on their 
      specialized migratory and tissue homing properties, the expression of chemokines 
      and their receptors along with the migratory responsiveness to chemokines of MoDC 
      in our study suggests a potential role of chemokines in the interaction between 
      dendritic cells and T cells and the induction of immune responses.
FAU - Zhu, K
AU  - Zhu K
AD  - Department of Dermatology, Second Affiliated Hospital, Zhejiang University, 
      Hangzhou 310009, China.
FAU - Shen, Q
AU  - Shen Q
FAU - Ulrich, M
AU  - Ulrich M
FAU - Zheng, M
AU  - Zheng M
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, CCR5)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, Interleukin-8A)
RN  - 0 (Receptors, Interleukin-8B)
SB  - IM
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/pharmacology
MH  - Chemokine CCL5/genetics/pharmacology
MH  - Chemokines/*genetics/pharmacology
MH  - Chemotaxis/drug effects
MH  - Dendritic Cells/cytology/immunology/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression
MH  - Humans
MH  - Interleukin-8/genetics/pharmacology
MH  - Monocytes/cytology
MH  - RNA, Messenger/genetics
MH  - Receptors, CCR2
MH  - Receptors, CCR5
MH  - Receptors, Chemokine/*genetics
MH  - Receptors, Interleukin-8A/genetics
MH  - Receptors, Interleukin-8B/genetics
EDAT- 2002/01/05 10:00
MHDA- 2002/01/31 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/01/31 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2000 Dec;113(12):1124-8.