PMID- 11776191
OWN - NLM
STAT- MEDLINE
DCOM- 20020130
LR  - 20131121
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 35
IP  - 7
DP  - 2000 Jul
TI  - [The effect of progesterone on proliferation and apoptosis in ovarian cancer 
      cell].
PG  - 423-6
AB  - OBJECTIVE: To investigate the regulatory effect of progesterone on proliferation 
      and apoptosis in ovarian cancer cell line HO8910 in vitro. METHODS: Ovarian 
      cancer cell line HO8910 originated from human ovarian serous cystadenocarcinoma 
      was cultured in vitro. Two groups were set up: study group (progesterone in 
      different concentrations) and control group without progesterone. Cell 
      proliferation was measured by 3-(4, 5-dimethylthiazol-z-yl)-2,5-dipheny 
      tetrazolium blue (MTT) colorimetric assay. Cell cycle and apoptotic percentage 
      were detected by flow cytometry, morphological changes of apoptotic cells were 
      observed by light and electron microscopy, and apoptotic cells were 
      quantitatively determined by terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL). In addition, expression of intracellular bcl-2 protein 
      was analyzed by flow cytometric indirect immunofluorescent technique. RESULTS: 
      Progesterone of 1 x 10(-7)-1 x 10(-5) mol/L inhibited HO8910 cell growth 
      significantly in a dose-dependent manner (P < 0.01). After treatment with 
      progesterone, the enhanced G0/G1 arrest was accompanied with the enhanced 
      apoptotic peak and percentage, as well apoptotic cells were found more than those 
      in control group (P < 0.05). By light and electron microscopy, there were many 
      morphological characteristics of apoptosis including compaction and margination 
      of nuclear chromatin, nuclear fragments, and apoptotic bodies. Analysis on 
      expression of intracellular bcl-2 protein showed that progesterone could 
      down-regulate bcl-2 protein and at concentration of 1 x 10(-5) mol/L it could 
      almost block bcl-2 expression. CONCLUSIONS: It is suggested in the present study 
      that progesterone can inhibit the proliferation of epithelial ovarian cancer 
      cells in vitro and there is an accordant dose-response relationship. Its 
      anticancer effect seems to be due to induction of apoptosis which maybe a result 
      of down-regulation of the anti-apoptotic protein bcl-2.
FAU - Hu, Z
AU  - Hu Z
AD  - Department of Obstetrics and Gynecology, First University Hospital, Chongqing 
      Medical University, Chongqing 400016, China.
FAU - Deng, X
AU  - Deng X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Cell Division/drug effects
MH  - Female
MH  - Humans
MH  - Ovarian Neoplasms/*pathology
MH  - Progesterone/*pharmacology
MH  - Proto-Oncogene Proteins c-bcl-2/analysis
MH  - Tumor Cells, Cultured
EDAT- 2002/01/05 10:00
MHDA- 2002/01/31 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/01/31 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2000 Jul;35(7):423-6.