PMID- 11776198
OWN - NLM
STAT- MEDLINE
DCOM- 20020130
LR  - 20101118
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 35
IP  - 8
DP  - 2000 Aug
TI  - [Detecting embryo sex by fluorescence in-situ hybridization in preimplantation 
      genetic diagnosis].
PG  - 465-7
AB  - OBJECTIVE: Applying fluorescence in situ hybridization(FISH) in preimplantation 
      gender diagnosis. METHODS: Ovarian hyperstimulation was performed in 2 hemophilia 
      A carriers and 2 patients with Y chromosome abnormality. Embryo sex was 
      identified by single blastomere FISH after embryo biopsy. Female embryos were 
      transferred into uterus. RESULTS: A total of 110 cumulus-oocyte complex were 
      retrieved in 5 treatment cycles. Among them, 68.2% showed normal fertilized 
      pronuclei and 55 embryos were available for embryo biopsy. The success rate of 
      biopsy was 85.5%, with further cleavage rate of 61.7%. In FISH procedure, one 
      cell was lost during fixation, led to 97.9% fixation rate. Totally, 18 female 
      embryos were diagnosed and 16 were transferred into uterus. Two clinical and one 
      biochemical pregnancies were achieved. The diagnosis was confirmed by the 
      following analysis of aminocyte and embryonic buds after embryo reduction 
      respectively. CONCLUSIONS: FISH is an efficient and accurate technique for 
      determination of the sex of human preimplantation embryos. Selective abortion and 
      birth of affected child can be avoided by preimplantation gender diagnosis.
FAU - Xu, Y
AU  - Xu Y
AD  - Reproductive Medical Research Center, Department of Obstetric and Gynecology, 
      First Affiliated Hospital of Sun Yat-sen University of Medical Sciences, 
      Guangzhou 510080, China.
FAU - Zhuang, G
AU  - Zhuang G
FAU - Li, M
AU  - Li M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
SB  - IM
MH  - Female
MH  - Fertilization in Vitro
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Pregnancy
MH  - *Preimplantation Diagnosis
MH  - *Sex Determination Analysis
EDAT- 2002/01/05 10:00
MHDA- 2002/01/31 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/01/31 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2000 Aug;35(8):465-7.