PMID- 11776402
OWN - NLM
STAT- MEDLINE
DCOM- 20020701
LR  - 20171116
IS  - 0171-2985 (Print)
IS  - 0171-2985 (Linking)
VI  - 204
IP  - 4
DP  - 2001 Dec
TI  - Enhanced contact hypersensitivity in human monocyte chemoattractant protein-1 
      transgenic mouse.
PG  - 477-93
AB  - Monocyte chemoattractant protein (MCP)-1 is a chemotactic cytokine for monocytes, 
      memoryT cells and dendritic cells (DC). However, the precise role of MCP-1 in a 
      variety of immunological responses remains unclear. In the present study, we 
      analyzed contact hypersensitivity (CHS) using human MCP-1 transgenic mice 
      (hMCP-1Tgm) that constitutively produce high levels of hMCP-1 in the sera. 
      Following 2,4-dinitrofluorobenzene (DNFB) sensitization, enhancement of CHS was 
      demonstrated in Tgm as compared with that in non-Tgm. Anti-hMCP-1 antibodies 
      significantly inhibited the CHS in Tgm. A prominent accumulation of B7-1+I-Ad+ 
      Langerhans' cells (LC) bearing haptens was detected in draining lymph nodes (DLN) 
      of Tgm 24 h after DNFB or fluorescein isothiocyanate (FITC) sensitization. 
      Similar results were obtained with BALB/c mice administrated recombinant (r) 
      hMCP-1. Langerhans' cells (LC) in the epidermal sheets of Tgm increased in size 
      and expressed high levels of I-Ad and B7-1 12 h after FITC application compared 
      with those of non-Tgm. After 18 h, the number of LC in the epidermis was reduced 
      in Tgm. It was also shown that the B7-1 expression on LC of BALB/c mice was 
      augmented after culture with rhMCP-1. These findings demonstrate that MCP-1 not 
      only accelerates LC migration from epidermis into the DLN after sensitization 
      with haptens but also up-regulates the I-Ad and B7-1 expressions, which results 
      in the enhanced T cell activation and CHS.
FAU - Mizumoto, N
AU  - Mizumoto N
AD  - Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University, 
      Sapporo, Japan.
FAU - Iwabichi, K
AU  - Iwabichi K
FAU - Nakamura, H
AU  - Nakamura H
FAU - Ato, M
AU  - Ato M
FAU - Shibaki, A
AU  - Shibaki A
FAU - Kawashima, T
AU  - Kawashima T
FAU - Kobayashi, H
AU  - Kobayashi H
FAU - Iwabuchi, C
AU  - Iwabuchi C
FAU - Ohkawara, A
AU  - Ohkawara A
FAU - Onoe, K
AU  - Onoe K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (B7-1 Antigen)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Haptens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (I-Ad antigen)
RN  - D241E059U6 (Dinitrofluorobenzene)
SB  - IM
MH  - Animals
MH  - B7-1 Antigen/immunology
MH  - Cell Division
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*immunology
MH  - Dendritic Cells/immunology
MH  - Dermatitis, Contact/*immunology
MH  - Dinitrofluorobenzene/immunology/pharmacology
MH  - Haptens/immunology
MH  - Histocompatibility Antigens Class II/immunology
MH  - Humans
MH  - Keratinocytes/cytology/immunology
MH  - Kinetics
MH  - Lymph Nodes/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Transgenic
MH  - Monocytes/immunology
MH  - T-Lymphocytes/cytology
MH  - Up-Regulation
EDAT- 2002/01/05 10:00
MHDA- 2002/07/02 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/07/02 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - S0171-2985(04)70033-9 [pii]
AID - 10.1078/0171-2985-00057 [doi]
PST - ppublish
SO  - Immunobiology. 2001 Dec;204(4):477-93. doi: 10.1078/0171-2985-00057.