PMID- 11777569 OWN - NLM STAT- MEDLINE DCOM- 20020228 LR - 20191105 IS - 0167-7012 (Print) IS - 0167-7012 (Linking) VI - 48 IP - 2-3 DP - 2002 Feb TI - Time-of-flight secondary ion mass spectrometry: characterisation of stainless steel surfaces immersed in natural seawater. PG - 195-205 AB - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has been employed to study the biofouling of stainless steel samples immersed in seawater. The aim of these characterisations was to understand the initial mechanisms of biomolecule adsorption for relatively short immersion times (from 0 to 24 h). The results show that: (i) there were unavoidable sample "precontaminations" on the surfaces, despite precaution during their preparation and manipulation (washing, drying and storing); (ii) the major peaks detected were the substrate ones whatever the immersion time [However, some organic (nitrogen and oxygen containing) and inorganic secondary ions appeared and grew with the immersion time.]; (iii) the surface contaminations, the nonuniformity of the adsorbed material so as and bacteria have been clearly observed by high-lateral resolution molecular ToF-SIMS mapping. FAU - Poleunis, C AU - Poleunis C AD - Unite de Physico-Chimie et de Physique des Materiaux (PCPM), Universite Catholique de Louvain (UCL), Croix du Sud 1, B-1348 Louvain-la-Neuve, Belgium. poleunis@pcpm.ucl.ac.be FAU - Compere, C AU - Compere C FAU - Bertrand, P AU - Bertrand P LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - J Microbiol Methods JT - Journal of microbiological methods JID - 8306883 RN - 0 (Proteins) RN - 12597-68-1 (Stainless Steel) SB - IM MH - Bacteria/*chemistry/isolation & purification MH - Proteins/*analysis MH - Seawater MH - *Spectrometry, Mass, Secondary Ion MH - *Stainless Steel MH - *Water Microbiology EDAT- 2002/01/05 10:00 MHDA- 2002/03/01 10:01 CRDT- 2002/01/05 10:00 PHST- 2002/01/05 10:00 [pubmed] PHST- 2002/03/01 10:01 [medline] PHST- 2002/01/05 10:00 [entrez] AID - S0167701201003232 [pii] AID - 10.1016/s0167-7012(01)00323-2 [doi] PST - ppublish SO - J Microbiol Methods. 2002 Feb;48(2-3):195-205. doi: 10.1016/s0167-7012(01)00323-2.