PMID- 11778695
OWN - NLM
STAT- MEDLINE
DCOM- 20020823
LR  - 20191025
IS  - 0920-8569 (Print)
IS  - 0920-8569 (Linking)
VI  - 23
IP  - 3
DP  - 2001 Dec
TI  - Gene expression analyzed by microarrays in HSV-1 latent mouse trigeminal ganglion 
      following heat stress.
PG  - 273-80
AB  - An understanding of the cellular genes whose expression is altered during HSV 
      reactivation will enable us to better understand host responses and biochemical 
      pathways involved in the process. Furthermore, this knowledge could allow us to 
      develop gene-targeted inhibitors to prevent viral reactivation. Mice latent with 
      HSV-1 strain McKrae and uninfected control mice were subjected to hyperthermic 
      stress (43 degrees C for 10 min) and their trigeminal ganglia (TG) collected 1 h 
      later. Two additional groups included HSV-1 latently infected and uninfected mice 
      not subjected to hyperthermic stress. Poly A+ mRNA was enriched from total mouse 
      TG RNA and reverse transcribed using MMLV RT. Radioactively labeled cDNAs were 
      analyzed by microarray analysis. A stress/toxicology array of 149 mouse genes on 
      a nylon membrane was used. The labeled cDNAs prepared from latently infected, 
      stressed mice demonstrated 3-fold or greater increases in certain mRNA-early 
      response genes (ERGs) compared to cDNAs from uninfected, stressed control mice. 
      The ERG mRNAs that showed increases included two heat shock proteins (HSP60 and 
      HSP40), a basic transcription factor (BTF T62), a DNA repair enzyme, two kinases 
      [MAP kinase and a stress-induced protein kinase (SADK)], an oxidative 
      stress-induced protein, a manganese superoxide dismutase precursor-2 (SOD-2), and 
      cyclooxygenase 2 (COX-2). The gene expression in unstressed, infected TGs was 
      similar to the gene expression in unstressed, uninfected controls. These results 
      suggest that there is a significant difference in the ERG expression profile in 
      latently infected TGs undergoing stress-induced reactivation compared to 
      uninfected TGs.
FAU - Hill, J M
AU  - Hill JM
AD  - Department of Ophthalmology (LSU Eye Center), New Orleans, LA 70112, USA. 
      jhill@lsuhsc.edu
FAU - Lukiw, W J
AU  - Lukiw WJ
FAU - Gebhardt, B M
AU  - Gebhardt BM
FAU - Higaki, S
AU  - Higaki S
FAU - Loutsch, J M
AU  - Loutsch JM
FAU - Myles, M E
AU  - Myles ME
FAU - Thompson, H W
AU  - Thompson HW
FAU - Kwon, B S
AU  - Kwon BS
FAU - Bazan, N G
AU  - Bazan NG
FAU - Kaufman, H E
AU  - Kaufman HE
LA  - eng
GR  - P30EY02377/EY/NEI NIH HHS/United States
GR  - R01AG18031/AG/NIA NIH HHS/United States
GR  - R01EY02672/EY/NEI NIH HHS/United States
GR  - R01EY05121/EY/NEI NIH HHS/United States
GR  - R01EY06311/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
SB  - IM
MH  - Animals
MH  - Female
MH  - *Gene Expression
MH  - Gene Expression Profiling
MH  - Herpesvirus 1, Human/*genetics/physiology
MH  - Hyperthermia, Induced
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oligonucleotide Array Sequence Analysis
MH  - Trigeminal Ganglion/*metabolism
MH  - Virus Activation
MH  - *Virus Latency
EDAT- 2002/01/10 10:00
MHDA- 2002/08/24 10:01
CRDT- 2002/01/10 10:00
PHST- 2002/01/10 10:00 [pubmed]
PHST- 2002/08/24 10:01 [medline]
PHST- 2002/01/10 10:00 [entrez]
AID - 10.1023/a:1012517221937 [doi]
PST - ppublish
SO  - Virus Genes. 2001 Dec;23(3):273-80. doi: 10.1023/a:1012517221937.