PMID- 11779134
OWN - NLM
STAT- MEDLINE
DCOM- 20020225
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 290
IP  - 1
DP  - 2002 Jan 11
TI  - Analysis of the gene expression profiles of immature versus mature bone 
      marrow-derived dendritic cells using DNA arrays.
PG  - 66-72
AB  - Dendritic cells (DCs) are professional antigen-presenting cells of the immune 
      system and can be generated in vitro from bone-marrow cells. In this study, we 
      systematically investigated by DNA array analysis the expression profiles of 514 
      immunologically relevant genes in two populations of mouse bone marrow-derived 
      DC, immature (DC(IMAT)), and lipopolysaccharide (LPS)-stimulated mature (DC(MAT)) 
      DCs. Our data showed that DC(IMAT) expressed transcripts for 69 (13.42% of the 
      514) of these genes and that, upon maturation, 32 (6.23%) of these were 
      up-regulated and 40 (7.78%) down-regulated. Maturation-dependent up-regulation, 
      defined by a differential expression (DE) ratio of >2, was observed among five 
      cytokine (Flt-3L, TNF-alpha, IL-1alpha and -1beta, and IL-6), three chemokine 
      (RANTES, MIP-2 and GROa) and three other (iNOS, MMP-13, and STRAP) genes. 
      Reciprocally, maturation-dependent down-regulation occurred with one cytokine 
      (IGF-1), two chemokine receptor (CCR2 and CCR5), and three other (RP105, Ax1, and 
      UCP2) genes. Lower level, but nevertheless significantly enhanced expression of 
      the chemokine receptor CCR7 and of NF-kappaB was also observed upon DC 
      maturation. This DC maturation profile confirms previous findings from other lab, 
      but it also substantially broadens our view of these cells by documenting 
      expression changes among genes (e.g., IGF-1, MMP-13, STRAP) not reported 
      previously in these cells.
CI  - (c)2002 Elsevier Science.
FAU - Chen, Zhuang
AU  - Chen Z
AD  - Research Unit, Saskatchewan Cancer Agency, 20 Campus Drive, Saskatoon, 
      Saskatchewan, S7N 4H4, Canada.
FAU - Gordon, John R
AU  - Gordon JR
FAU - Zhang, Xueshu
AU  - Zhang X
FAU - Xiang, Jim
AU  - Xiang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - Bone Marrow Cells/*metabolism
MH  - Cell Movement
MH  - Chemokines/biosynthesis
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/*metabolism
MH  - Down-Regulation
MH  - Flow Cytometry
MH  - Immunophenotyping
MH  - Lipopolysaccharides/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Phenotype
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Signal Transduction
MH  - Time Factors
MH  - Up-Regulation
EDAT- 2002/01/10 10:00
MHDA- 2002/02/28 10:01
CRDT- 2002/01/10 10:00
PHST- 2002/01/10 10:00 [pubmed]
PHST- 2002/02/28 10:01 [medline]
PHST- 2002/01/10 10:00 [entrez]
AID - S0006291X01961479 [pii]
AID - 10.1006/bbrc.2001.6147 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2002 Jan 11;290(1):66-72. doi: 
      10.1006/bbrc.2001.6147.