PMID- 11779135
OWN - NLM
STAT- MEDLINE
DCOM- 20020225
LR  - 20111117
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 290
IP  - 1
DP  - 2002 Jan 11
TI  - Differential expression of the isoforms for the monocyte chemoattractant 
      protein-1 receptor, CCR2, in monocytes.
PG  - 73-80
AB  - Two isoforms of human CCR2, the receptor for monocyte chemoattractant protein-1 
      (MCP-1), have been identified but their relative expression in monocytes and 
      contribution to inflammatory responses mediated by MCP-1 remain uncertain. All 
      available information on CCR2 expression is based on mRNA data because 
      isoform-specific antibodies were not available until now. To analyze the relative 
      expression of each isoform, we made two antibodies that specifically recognized 
      CCR2A and CCR2B. Examination of receptor protein with these isoform-specific 
      antibodies showed that the total expression of CCR2B in monocytes was about 
      10-fold higher than that of CCR2A with an equal distribution between the cell 
      surface and intracellular pools. A detailed analysis using purified plasma 
      membranes demonstrated that about 90% of all CCR2 on the cell surface were 
      composed of CCR2B. The relatively abundant expression of CCR2B on the cell 
      surface suggests a principal role of this isoform as a mediator of monocyte 
      responses to MCP-1 in inflammation.
CI  - (c)2002 Elsevier Science.
FAU - Tanaka, Seiya
AU  - Tanaka S
AD  - Department of Medicine, University of California San Diego, 9500 Gilman Drive, La 
      Jolla, CA 92093-0682, USA.
FAU - Green, Simone R
AU  - Green SR
FAU - Quehenberger, Oswald
AU  - Quehenberger O
LA  - eng
GR  - HL 56989/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CCR2 protein, human)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Cell Membrane/metabolism
MH  - Cells, Cultured
MH  - Flow Cytometry
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/metabolism
MH  - Humans
MH  - Inflammation
MH  - Luminescent Measurements
MH  - Monocytes/metabolism
MH  - Protein Binding
MH  - Protein Isoforms
MH  - RNA, Messenger/metabolism
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/*chemistry/*immunology
MH  - Signal Transduction
MH  - Transfection
EDAT- 2002/01/10 10:00
MHDA- 2002/02/28 10:01
CRDT- 2002/01/10 10:00
PHST- 2002/01/10 10:00 [pubmed]
PHST- 2002/02/28 10:01 [medline]
PHST- 2002/01/10 10:00 [entrez]
AID - S0006291X01961492 [pii]
AID - 10.1006/bbrc.2001.6149 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2002 Jan 11;290(1):73-80. doi: 
      10.1006/bbrc.2001.6149.