PMID- 11782467
OWN - NLM
STAT- MEDLINE
DCOM- 20020405
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 277
IP  - 11
DP  - 2002 Mar 15
TI  - p53 Transcriptional activity is mediated through the SRC1-interacting domain of 
      CBP/p300.
PG  - 9054-61
AB  - The tumor suppressor p53 recruits the cellular coactivator CBP/p300 to mediate 
      the transcriptional activation of target genes. In this study, we identify a 
      novel p53-interacting region in CBP/p300, which we call CR2, located near the 
      carboxyl terminus. The 95-amino acid CR2 region (amino acids 2055--2150) is 
      located adjacent to the C/H3 domain and corresponds precisely with the minimal 
      steroid receptor coactivator 1 (SRC1)-interacting domain of CBP (also called 
      IBiD). We show that the region of p53 that participates in the CR2 interaction 
      resides within the first 107 amino acids of the protein. p53 binds strongly to 
      the CR2 domain of both CBP and the highly homologous coactivator p300. 
      Importantly, an in-frame deletion of CR2 within the full-length p300 protein 
      strongly compromises p300-mediated p53 transcriptional activation from a 
      chromatin template in vitro. The identification of the p53-interacting CR2 domain 
      in CBP/p300 prompted us to ask if the human T-cell leukemia virus (HTLV-I) Tax 
      protein, which also interacts with CR2, competes with p53 for binding to this 
      domain. We show that p53 and Tax exhibit mutually exclusive binding to the CR2 
      region, possibly contributing to the previously reported Tax repression of p53 
      function. Together, these studies identify and molecularly characterize a new p53 
      binding site on CBP/p300 that participates in coactivator-mediated p53 
      transcription function. The identity of the p53.CR2 interaction indicates that at 
      least three distinct sites on CBP/p300 may participate in mediating p53 
      transactivation.
FAU - Livengood, Jill A
AU  - Livengood JA
AD  - Department of Biochemistry and Molecular Biology, Colorado State University, Fort 
      Collins, Colorado 80523-1870, USA.
FAU - Scoggin, Kirsten E S
AU  - Scoggin KE
FAU - Van Orden, Karen
AU  - Van Orden K
FAU - McBryant, Steven J
AU  - McBryant SJ
FAU - Edayathumangalam, Rajeswari S
AU  - Edayathumangalam RS
FAU - Laybourn, Paul J
AU  - Laybourn PJ
FAU - Nyborg, Jennifer K
AU  - Nyborg JK
LA  - eng
GR  - R01 CA055035/CA/NCI NIH HHS/United States
GR  - R01 CA055035-08/CA/NCI NIH HHS/United States
GR  - CA-55035/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20020108
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Gene Products, tax)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (NCOA1 protein, human)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
SB  - IM
MH  - Binding Sites
MH  - Binding, Competitive
MH  - Cell Transformation, Neoplastic
MH  - Gene Products, tax/metabolism
MH  - Histone Acetyltransferases
MH  - Humans
MH  - Jurkat Cells
MH  - Nuclear Proteins/chemistry/*physiology
MH  - Nuclear Receptor Coactivator 1
MH  - Trans-Activators/chemistry/*physiology
MH  - Transcription Factors/*metabolism
MH  - *Transcriptional Activation
MH  - Tumor Suppressor Protein p53/chemistry/*physiology
EDAT- 2002/01/10 10:00
MHDA- 2002/04/06 10:01
CRDT- 2002/01/10 10:00
PHST- 2002/01/10 10:00 [pubmed]
PHST- 2002/04/06 10:01 [medline]
PHST- 2002/01/10 10:00 [entrez]
AID - S0021-9258(19)36270-2 [pii]
AID - 10.1074/jbc.M108870200 [doi]
PST - ppublish
SO  - J Biol Chem. 2002 Mar 15;277(11):9054-61. doi: 10.1074/jbc.M108870200. Epub 2002 
      Jan 8.