PMID- 11783013
OWN - NLM
STAT- MEDLINE
DCOM- 20020226
LR  - 20081121
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 23
IP  - 2
DP  - 2001 Mar
TI  - [Experimental study of anti-metastasis effect of urokinase amino-terminal 
      fragment gene on human breast cancer cells].
PG  - 115-7
AB  - OBJECTIVE: To explore the suppressive effects of urokinase amino-terminal 
      fragment (ATF) gene on metastatic potential of human breast cancer cell line 
      MCF-7. METHODS: A pcDNA3-ATF plasmid containing ATF cDNA under CMV 
      promotor/enhancer control was constructed and transfected into MCF-7 cells by 
      lipofectin. The expression of of uPA/uPAR and ATF in MCF-7 cells were analyzed by 
      RT-PCR and Western blot. The effect of ATF expression on invasiveness in vitro, 
      tumorigenesis and metastasis in vivo of MCF-7 cell was investigated. RESULTS: 
      MCF-7 cells displayed an overexpression of uPA/uPAR. Expression of ATF was 
      detected after ATF gene-transfection. The invasive capacity of ATF 
      gene-transfected MCF-7 cells was decreased significantly. Although the 
      tumorigenesis was not affected, the in vivo metastasis of ATF gene-transfected 
      MCF-7 cells was remarkably inhibited. CONCLUSION: Suppression of invasiveness and 
      metastasis of ATF-transfected MCF-7 cells is perhaps due to a competitive 
      inhibition of interaction with endogenous uPA/uPAR.
FAU - Zhu, F
AU  - Zhu F
AD  - Beijing Institute of Radiation Medicine, Beijing 100850, China.
FAU - Xing, G
AU  - Xing G
FAU - He, F
AU  - He F
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
RN  - 0 (DNA, Complementary)
RN  - 0 (PLAUR protein, human)
RN  - 0 (Peptide Fragments)
RN  - 0 (Plaur protein, mouse)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Breast Neoplasms/genetics/pathology/*therapy
MH  - DNA, Complementary/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Neoplasm Invasiveness
MH  - Neoplasm Metastasis/genetics/*prevention & control
MH  - Neoplasm Transplantation
MH  - Peptide Fragments/genetics
MH  - Receptors, Cell Surface/genetics
MH  - Receptors, Urokinase Plasminogen Activator
MH  - Recombinant Fusion Proteins/genetics
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Urokinase-Type Plasminogen Activator/chemistry/*genetics
MH  - Xenograft Model Antitumor Assays
EDAT- 2002/01/11 10:00
MHDA- 2002/02/28 10:01
CRDT- 2002/01/11 10:00
PHST- 2002/01/11 10:00 [pubmed]
PHST- 2002/02/28 10:01 [medline]
PHST- 2002/01/11 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2001 Mar;23(2):115-7.