PMID- 11783350
OWN - NLM
STAT- MEDLINE
DCOM- 20020117
LR  - 20061115
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 36
IP  - 2
DP  - 2001 Feb
TI  - [Application of inter-fluorescence in situ hybridization of chromosome 13/21 
      alpha satellite probe in amniotic cells for prenatal diagnosis trisomy 21 
      syndrome].
PG  - 76-8
AB  - OBJECTIVE: To investigate the prenatal diagnosis of trisomy 21 syndrome using 
      chromosome 13/21 alpha satellite probe fluorescence in situ hybridization (FISH) 
      on uncultured interphase cells from amniotic fluid. METHODS: The interphase 
      amniocytes of 10 fetuses who were detected normal and 3 fetus who were detected 
      trisomy by prenatal cytogenetic diagnosis were selected. We did FISH which used 
      chromosome 13/21 alpha satellite probe directly on the uncultured amniocytes of 
      these 13 samples. RESULTS: The total rate of the hybridization was 36.7% and 
      38.6% in control group and observation group respectively, showed no 
      significantly difference. There were four signals in the nucleus, two groups were 
      36.5% and 3.9% respectively, there were five signals in the nucleus, two groups 
      were 4.0% and 36.1% respectively. The control group and observation group showed 
      significantly difference by the statistical chi 2 values (P < 0.01). Trisomy 21 
      syndrome was diagnosed when nucleus of five signals accounted for more than 
      36.1%. CONCLUSION: FISH with Chromosome 13/21 alpha satellite probe is a valuable 
      method for rapid prenatal diagnosis of trisomy 21 syndrome.
FAU - Li, W
AU  - Li W
AD  - Institute of Medical Genetics Zhuhai, Guangdong Province, Zhuhai 519000, China.
FAU - Wu, Y
AU  - Wu Y
FAU - Ye, Z
AU  - Ye Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Amniotic Fluid/*cytology
MH  - Chromosomes, Human, Pair 13/*genetics
MH  - DNA Probes
MH  - DNA, Satellite
MH  - Down Syndrome/*diagnosis
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
EDAT- 2002/01/11 10:00
MHDA- 2002/01/23 10:01
CRDT- 2002/01/11 10:00
PHST- 2002/01/11 10:00 [pubmed]
PHST- 2002/01/23 10:01 [medline]
PHST- 2002/01/11 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2001 Feb;36(2):76-8.