PMID- 11785875
OWN - NLM
STAT- MEDLINE
DCOM- 20020910
LR  - 20031114
IS  - 0005-2086 (Print)
IS  - 0005-2086 (Linking)
VI  - 45
IP  - 4
DP  - 2001 Oct-Dec
TI  - Efficacy and safety of an infectious bursal disease virus intermediate vaccine in 
      ovo.
PG  - 1036-43
AB  - The study was divided into two experiments. In the first experiment, the efficacy 
      of in ovo intermediate vaccine against infectious bursal disease virus (IBDV) was 
      determined by challenge at 21 days of age with virulent IBDV in 
      specific-pathogen-free (SPF) and commercial chickens. This vaccine was able to 
      induce active immunity and to protect SPF chickens to challenge; protection was 
      not complete in commercial chickens, as testified by bursal lesions, bursal index 
      after challenge, and vaccine immunoresponse. In order to detect field and 
      vaccinal viruses, immunoperoxidase staining, enzyme-linked immunosorbent assay, 
      capture, and reverse transcriptase-polymerase chain reaction (RT-PCR) were 
      tested; the RT-PCR was more effective at detecting both kind of viruses. In the 
      second experiment, the immunosuppressive effect of in ovo vaccination was 
      determined by evaluating the immunoresponse against Newcastle disease virus (NDV) 
      vaccination effected at 10 days in both SPF and commercial chickens vaccinated in 
      ovo. The in ovo vaccine causes a reduction of NDV immunoresponse, as testified by 
      lowest geometric mean titer in group I (SPF chickens vaccinated against IBDV in 
      ovo and against NDV at 11 days). In commercial chickens, immunoresponse to NDV 
      vaccination was not influenced by in ovo vaccination.
FAU - Coletti, M
AU  - Coletti M
AD  - Dipartimento di Scienze Biopatologiche Veterinarie, Universita degli Studi di 
      Perugia, Italy.
FAU - Del Rossi, E
AU  - Del Rossi E
FAU - Franciosini, M P
AU  - Franciosini MP
FAU - Passamonti, F
AU  - Passamonti F
FAU - Tacconi, G
AU  - Tacconi G
FAU - Marini, C
AU  - Marini C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Avian Dis
JT  - Avian diseases
JID - 0370617
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Birnaviridae Infections/prevention & control/*veterinary
MH  - Bursa of Fabricius/pathology/virology
MH  - Chick Embryo
MH  - *Chickens
MH  - Infectious bursal disease virus/*immunology/pathogenicity
MH  - Newcastle Disease/prevention & control
MH  - Newcastle disease virus/immunology
MH  - Poultry Diseases/*prevention & control
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Safety
MH  - Specific Pathogen-Free Organisms
MH  - Treatment Outcome
MH  - Viral Vaccines/immunology/*standards
MH  - Virulence
EDAT- 2002/01/12 10:00
MHDA- 2002/09/11 10:01
CRDT- 2002/01/12 10:00
PHST- 2002/01/12 10:00 [pubmed]
PHST- 2002/09/11 10:01 [medline]
PHST- 2002/01/12 10:00 [entrez]
PST - ppublish
SO  - Avian Dis. 2001 Oct-Dec;45(4):1036-43.