PMID- 11788470
OWN - NLM
STAT- MEDLINE
DCOM- 20020207
LR  - 20190901
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Linking)
VI  - 22
IP  - 1
DP  - 2002 Jan
TI  - Local overexpression of monocyte chemoattractant protein-1 at vessel wall induces 
      infiltration of macrophages and formation of atherosclerotic lesion: synergism 
      with hypercholesterolemia.
PG  - 115-20
AB  - Monocyte/macrophage infiltration to the arterial wall is an initial step in 
      atherosclerosis, and monocyte chemoattractant protein-1 (MCP-1) is thought to 
      play a central role in the recruitment of these cells. In the present study, we 
      examined the role of local expression of MCP-1 at the vessel wall in the 
      initiation and development of atherosclerosis. We transfected the cDNA encoding 
      rat MCP-1 into the vessel wall of the rabbit carotid artery with the use of the 
      hemagglutinating virus of Japan (HVJ)-liposome method. The rabbits were divided 
      into the following groups: (1) those fed normal chow and transfected with 
      MCP-1-HVJ, (2) those fed a high cholesterol diet (1% cholesterol) and transfected 
      with MCP-1-HVJ, and (3) those fed a high cholesterol diet and transfected with 
      control-HVJ. Prescribed diets were started 2 weeks before transfection and were 
      continued for another 2 weeks. In group 1, vascular lesion formation was not 
      found, and anti-rabbit monocyte/macrophage antibody (RAM-11) staining for 
      monocytes/macrophages was negative, although anti-rat MCP-1 antibody (R-17) 
      staining for rat MCP-1 was positive mainly in endothelial cells. Cholesterol 
      feeding increased plasma cholesterol levels to 1801+/-444 mg/dL in group 2. In 
      group 2, all rabbits displayed neointimal formation with infiltration of 
      RAM-11-positive cells, and a part of the lesion was also positive for Sudan III 
      lipid staining. In group 3, hypercholesterolemia did not induce the infiltration 
      of monocytes/macrophages and subsequent lesion formation in the vessel wall 
      despite definite upregulation of intercellular adhesion molecule-1 and vascular 
      cell adhesion molecule-1 on the endothelium. To initiate atherosclerotic changes, 
      local MCP-1 overexpression at the vessel is not sufficient, and activation of 
      other factors induced by hypercholesterolemia is required.
FAU - Namiki, Masayuki
AU  - Namiki M
AD  - Division of Cardiovascular and Respiratory Medicine, Kobe University Graduate 
      School of Medicine, Kobe, Japan.
FAU - Kawashima, Seinosuke
AU  - Kawashima S
FAU - Yamashita, Tomoya
AU  - Yamashita T
FAU - Ozaki, Masanori
AU  - Ozaki M
FAU - Hirase, Tetsuaki
AU  - Hirase T
FAU - Ishida, Tatsuro
AU  - Ishida T
FAU - Inoue, Nobutaka
AU  - Inoue N
FAU - Hirata, Ken-ichi
AU  - Hirata K
FAU - Matsukawa, Akihiro
AU  - Matsukawa A
FAU - Morishita, Ryuichi
AU  - Morishita R
FAU - Kaneda, Yasufumi
AU  - Kaneda Y
FAU - Yokoyama, Mitsuhiro
AU  - Yokoyama M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Arteriosclerosis/blood/etiology/*pathology
MH  - Carotid Artery Diseases/etiology
MH  - Cell Movement/*physiology
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Cholesterol/administration & dosage/blood
MH  - Diet, Atherogenic
MH  - Endothelium, Vascular/*pathology
MH  - Hypercholesterolemia/complications
MH  - Intercellular Adhesion Molecule-1/genetics/metabolism
MH  - Macrophages/*physiology
MH  - Male
MH  - Monocytes/*physiology
MH  - Rabbits
MH  - Rats
MH  - Transfection
MH  - Up-Regulation
MH  - Vascular Cell Adhesion Molecule-1/genetics/metabolism
EDAT- 2002/01/15 10:00
MHDA- 2002/02/08 10:01
CRDT- 2002/01/15 10:00
PHST- 2002/01/15 10:00 [pubmed]
PHST- 2002/02/08 10:01 [medline]
PHST- 2002/01/15 10:00 [entrez]
AID - 10.1161/hq0102.102278 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2002 Jan;22(1):115-20. doi: 10.1161/hq0102.102278.