PMID- 11788794
OWN - NLM
STAT- MEDLINE
DCOM- 20020515
LR  - 20191025
IS  - 0282-0080 (Print)
IS  - 0282-0080 (Linking)
VI  - 18
IP  - 4
DP  - 2001 Apr
TI  - Low-dose intragastric administration of Phaseolus vulgaris agglutinin (PHA) does 
      not induce immunoglobulin E (IgE) production in Sprague-Dawley rats.
PG  - 273-5
AB  - Native Phaseolus vulgaris agglutinin (PHA) poses a potential health threat, when 
      ingested with improperly cooked red kidney beans. Since PHA triggers human 
      basophilic granulocytes in culture to rapidly release considerable amounts of 
      interleukin-(IL-)4 and IL-13, key cytokines for inducing immunoglobulin E (IgE) 
      production, the question was addressed whether this lectin can evoke in vivo IgE 
      production. IgE-low-responder (Sprague-Dawley) rats received PHA (6 mg/rat/day) 
      intragastrically by gavage over a period of 10 days. Up to day 35, there was no 
      IgE induction regardless of whether the animals were boostered subcutaneously 
      with PHA or not, indicating that PHA cannot be regarded as a general IgE inducer 
      in rats.
FAU - Haas, H
AU  - Haas H
AD  - Forschungszentrum Borstel, D-23845 Borstel, Germany. hhaas@fz-borstel.de
FAU - Herzig, K H
AU  - Herzig KH
FAU - Andre, S
AU  - Andre S
FAU - Galle, J
AU  - Galle J
FAU - Gronow, A
AU  - Gronow A
FAU - Gabius, H J
AU  - Gabius HJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Glycoconj J
JT  - Glycoconjugate journal
JID - 8603310
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Plant Lectins)
RN  - 0 (Protein Isoforms)
RN  - 0 (leukoagglutinins, plants)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fabaceae/*immunology
MH  - Food Hypersensitivity/*immunology
MH  - Immunoglobulin E/*biosynthesis/immunology
MH  - Male
MH  - Models, Animal
MH  - Phytohemagglutinins/*administration & dosage/blood/*immunology
MH  - Plant Lectins
MH  - Protein Isoforms/immunology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
EDAT- 2002/01/15 10:00
MHDA- 2002/05/16 10:01
CRDT- 2002/01/15 10:00
PHST- 2002/01/15 10:00 [pubmed]
PHST- 2002/05/16 10:01 [medline]
PHST- 2002/01/15 10:00 [entrez]
AID - 10.1023/a:1013740413012 [doi]
PST - ppublish
SO  - Glycoconj J. 2001 Apr;18(4):273-5. doi: 10.1023/a:1013740413012.