PMID- 11788884
OWN - NLM
STAT- MEDLINE
DCOM- 20020405
LR  - 20210102
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 20
IP  - 2
DP  - 2002 Feb
TI  - Generation of dendritic cells from human bone marrow mononuclear cells: 
      advantages for clinical application in comparison to peripheral blood monocyte 
      derived cells.
PG  - 247-53
AB  - Dendritic cells (DCs) currently used for vaccination in clinical studies to 
      induce immunity against malignant cells are normally generated from peripheral 
      blood-derived monocytes. Here we studied conditions for the generation of DCs 
      from unseparated human bone marrow (BM) mononuclear cells and compared them 
      functionally with DCs from blood. The two types of DCs, from bone marrow (BM-DC) 
      and peripheral blood (BL-DC), were generated in parallel from the same normal 
      healthy donors by culturing in serum-free X-VIVO 20 medium containing GM-CSF and 
      IL-4, and then the phenotypes and functions were compared. BM-DC generation 
      occurred in 14 days and involved proliferative expansion from CD34 stem cells and 
      differentiation while BL-DC generation occurred in 7 days from CD14 monocytes and 
      involved only differentiation. A 7- to 25-fold higher number of DCs could be 
      obtained from BM than from blood. BM-DC had similar phenotypes as BL-DC. The 
      capacity to stimulate MLR reactivity in allogeneic T lymphocytes was higher with 
      BM-DC than that with BL-DC. Also, the capacity to stimulate autologous memory T 
      cell responses to tetanus toxoid (TT) or tuberculin (PPD) was higher with BM-DC 
      than with BL-DC. These results suggest that BM-DC as produced here may be a very 
      economic and useful source of professional antigen-presenting cells for 
      anti-tumor immunotherapeutic protocols.
FAU - Bai, L
AU  - Bai L
AD  - German Cancer Research Center, Division of Cellular Immunology, D-69120 
      Heidelberg, Germany.
FAU - Feuerer, M
AU  - Feuerer M
FAU - Beckhove, P
AU  - Beckhove P
FAU - Umansky, V
AU  - Umansky V
FAU - Schirrmacher, V
AU  - Schirrmacher V
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
SB  - IM
MH  - Antigen Presentation
MH  - Bone Marrow Cells/*cytology
MH  - *Cell Differentiation
MH  - Cell Size
MH  - Dendritic Cells/*cytology/physiology
MH  - Endocytosis
MH  - Flow Cytometry
MH  - Humans
MH  - Leukocytes, Mononuclear/*cytology
MH  - Lymphocyte Culture Test, Mixed
MH  - Phenotype
EDAT- 2002/01/15 10:00
MHDA- 2002/04/06 10:01
CRDT- 2002/01/15 10:00
PHST- 2002/01/15 10:00 [pubmed]
PHST- 2002/04/06 10:01 [medline]
PHST- 2002/01/15 10:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2002 Feb;20(2):247-53.