PMID- 11789735
OWN - NLM
STAT- MEDLINE
DCOM- 20020122
LR  - 20141120
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 117
IP  - 1
DP  - 2002 Jan
TI  - A formalin-fixed, paraffin-processed cell line standard for quality control of 
      immunohistochemical assay of HER-2/neu expression in breast cancer.
PG  - 81-9
AB  - To ensure the accuracy and reproducibility of immunohistochemical assays for 
      determining HER-2/neu status of patients with breast cancer, a reliable standard 
      for monitoring assay sensitivity is necessary. We optimally fixed and paraffin 
      processed human ovarian and breast carcinoma cell lines SKOV-3, MDA-MB-453, 
      BT-20, and MCF-7 in quantities sufficient to meet the needs of a laboratory for 
      the foreseeable future. The material was tested, alongside HercepTest kit cell 
      lines (DAKO, Carpinteria, CA), by 7 breast cancer centers in the United Kingdom 
      and France with different immunohistochemical assays and markers. The cell lines 
      also were analyzed by fluorescence in situ hybridization (FISH) by 2 centers 
      using HER-2/neu kits. FISH produced 100% agreement between the 2 centers: SKOV-3 
      and MDA-MB-453 showed HER-2/neu amplification and BT-20 and MCF-7 did not. 
      Immunohistochemical analysis and a common evaluation method produced 100% 
      agreement that SKOV-3 and MCF-7 showed 3+ and zero HER-2/neu overexpression, 
      respectively. For MDA-MB-453, there was 71% (5/7) concordance of 2+ 
      immunohistochemical staining and 86% (6/7) concordance of zero or 1 + staining 
      for BT-20. The cell lines provide a valuable standard for gauging HER-2/neu assay 
      sensitivity irrespective of the antibody, antigen retrieval system, detection 
      system, or method of evaluation used.
FAU - Rhodes, Anthony
AU  - Rhodes A
AD  - United Kingdom National External Quality Assessment Scheme for 
      Immunocytochemistry and the Department of Histopathology, University College 
      London Medical School, England.
FAU - Jasani, Bharat
AU  - Jasani B
FAU - Couturier, Jerjme
AU  - Couturier J
FAU - McKinley, Mark J
AU  - McKinley MJ
FAU - Morgan, John M
AU  - Morgan JM
FAU - Dodson, Andrew R
AU  - Dodson AR
FAU - Navabi, Hossein
AU  - Navabi H
FAU - Miller, Keith D
AU  - Miller KD
FAU - Balaton, Andre J
AU  - Balaton AJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 1HG84L3525 (Formaldehyde)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
CIN - Am J Clin Pathol. 2002 Jan;117(1):7-8. PMID: 11789733
MH  - Breast Neoplasms/immunology/*pathology
MH  - Female
MH  - Formaldehyde
MH  - Humans
MH  - *Immunohistochemistry/standards
MH  - In Situ Hybridization, Fluorescence
MH  - Paraffin Embedding/standards
MH  - Quality Control
MH  - Receptor, ErbB-2/*analysis
MH  - Reference Standards
MH  - Tissue Fixation/standards
MH  - Tumor Cells, Cultured
EDAT- 2002/01/16 10:00
MHDA- 2002/01/23 10:01
CRDT- 2002/01/16 10:00
PHST- 2002/01/16 10:00 [pubmed]
PHST- 2002/01/23 10:01 [medline]
PHST- 2002/01/16 10:00 [entrez]
AID - 10.1309/4NCM-QJ9W-QM0J-6QJE [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2002 Jan;117(1):81-9. doi: 10.1309/4NCM-QJ9W-QM0J-6QJE.