PMID- 11790349
OWN - NLM
STAT- MEDLINE
DCOM- 20020301
LR  - 20191210
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 125
IP  - 3
DP  - 2000 Mar
TI  - 3,3',4,4'-Tetrachlorobiphenyl oxidation in fish, bird and reptile species: 
      relationship to cytochrome P450 1A inactivation and reactive oxygen production.
PG  - 273-86
AB  - Previously we showed that the polychlorinated biphenyl 
      3,3',4,4'-tetrachlorobiphenyl (TCB) caused a release of reactive oxygen species 
      (ROS) from cytochrome P450 1A (CYP1A) of the fish scup (Stenotomus chrysops), and 
      from rat and human CYP1A1. This was linked to a TCB- and NADPH-dependent 
      oxidative inactivation of the enzyme, which in scup and rat was inversely related 
      to the rates of TCB oxidation. We examined the relationship between rates of TCB 
      oxidation, CYP1A inactivation and ROS production in liver microsomes from 
      additional vertebrate species, including skate (Raja erinacea), eel (Anguilla 
      rostrata), killifish (Fundulus heteroclitus), winter flounder (Pleuronectes 
      americanus), chicken (Gallus domesticus), cormorant (Phalacrocorax auritus), gull 
      (Larus argentatus), and turtle (Chrysemys picta picta). TCB oxidation rates were 
      induced in all fish and birds treated with aryl hydrocarbon receptor agonists. 
      Induced rates of TCB oxidation were <1 pmol/min/mg microsomal protein in all 
      fish, and 6-14 pmol/min/mg in the birds. In all species but one, TCB oxidation 
      rates correlated positively with EROD rates, indicating likely involvement of 
      CYP1A in TCB oxidation. Incubation of liver microsomes of most species with 
      TCB+NADPH resulted in an immediate (TCB-dependent) inhibition of EROD, and a 
      progressive loss of EROD capacity, indicating an oxidative inactivation of CYP1A 
      like that in scup. NADPH stimulated production of ROS (H(2)O(2) and/or O(2)(-*)) 
      by liver microsomes, slightly in some species (eel) and greatly in others 
      (chicken, turtle). Among the birds and the fish, NADPH-stimulated ROS production 
      correlated positively with EROD activity. TCB caused a significant stimulation of 
      ROS production by liver microsomes of flounder, killifish, cormorant and gull, as 
      well as scup. The stimulation of CYP1A inactivation and ROS generation indicates 
      an uncoupling of CYP1A by TCB in many species, and when compared between species, 
      the rates of CYP1A inactivation correlated inversely with rates of TCB oxidation. 
      Some feature(s) of binding/active site topology may hinder TCB oxidation, 
      enhancing the likelihood for attack of an oxidizing species in the active site.
FAU - Schlezinger, J J
AU  - Schlezinger JJ
AD  - Biology Department, Redfield 342, MS 32, Woods Hole Oceanographic Institution, 
      Woods Hole, MA 02543, USA.
FAU - Keller, J
AU  - Keller J
FAU - Verbrugge, L A
AU  - Verbrugge LA
FAU - Stegeman, J J
AU  - Stegeman JJ
LA  - eng
GR  - P42-ES07381/ES/NIEHS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 1364PS73AF (Acetone)
RN  - 53-59-8 (NADP)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - Y2I6546TMI (3,4,3',4'-tetrachlorobiphenyl)
SB  - IM
MH  - Acetone/pharmacology
MH  - Anguilla
MH  - Animals
MH  - Birds
MH  - Chickens
MH  - Cytochrome P-450 CYP1A1/drug effects/*metabolism
MH  - Female
MH  - Flounder
MH  - Fundulidae
MH  - Microsomes, Liver/drug effects/enzymology
MH  - NADP/pharmacology
MH  - Oxidation-Reduction
MH  - Polychlorinated Biphenyls/*metabolism
MH  - Reactive Oxygen Species/*metabolism
MH  - Receptors, Aryl Hydrocarbon/agonists/drug effects
MH  - Skates, Fish
MH  - Species Specificity
MH  - Turtles
EDAT- 2002/01/16 10:00
MHDA- 2002/03/02 10:01
CRDT- 2002/01/16 10:00
PHST- 2002/01/16 10:00 [pubmed]
PHST- 2002/03/02 10:01 [medline]
PHST- 2002/01/16 10:00 [entrez]
AID - S0742841399001127 [pii]
AID - 10.1016/s0742-8413(99)00112-7 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2000 Mar;125(3):273-86. doi: 
      10.1016/s0742-8413(99)00112-7.