PMID- 11790664
OWN - NLM
STAT- MEDLINE
DCOM- 20020221
LR  - 20071114
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 165
IP  - 2
DP  - 2002 Jan 15
TI  - Oligoclonal T cell expansions in pulmonary lymphoproliferative disorders: 
      demonstration of the frequent occurrence of oligoclonal T cells in human 
      immunodeficiency virus-related lymphoid interstitial pneumonia.
PG  - 254-9
AB  - We used a denaturing gradient gel electrophoresis (DGGE) procedure with 
      40-nucleotide guanine- and cytosine-rich sequences in the polymerase chain 
      reaction (PCR) and sequencing analysis to analyze the T cell antigen receptor 
      (TCR)-Vgamma gene repertoire of infiltrating T lymphocytes in pulmonary 
      lymphoproliferative disorders. Six of 15 low-grade mucosa-associated lymphoid 
      tissue (MALT) lymphomas and 8 of 15 cases of lymphocytic interstitial pneumonia 
      (LIP) showed some oligoclonal bands for TCR-Vgamma genes on DGGE. Sequencing 
      analysis demonstrated plural oligoclonal TCR-Vgamma clones among the oligoclonal 
      PCR products on DGGE, leading to the conclusion that conventional 
      antigen-specific oligoclonal expansions may play some role in the pathogenesis of 
      pulmonary lymphoproliferative disorders. The frequency of oligoclonal 
      infiltrating T cell expansions in human immunodeficiency virus (HIV)-related LIP 
      (100%) was significantly higher than in low-grade pulmonary MALT lymphomas (40%) 
      or in HIV-negative LIP (30%). Because recent evidence demonstrates that the V3 
      loop in the proviral amino acid sequences of mononuclear cells from 
      bronchoalveolar lavage is more homogeneous than those from peripheral blood, this 
      homogeneity might result in oligoclonal expansions of infiltrating T lymphocytes 
      as a consequence of ongoing reactions against lung-specific viral strains.
FAU - Kurosu, Katsushi
AU  - Kurosu K
AD  - Department of Respirology, School of Medicine, Chiba University, Chiba, Japan. 
      SNC16385@nifty.com
FAU - Yumoto, Norio
AU  - Yumoto N
FAU - Rom, William N
AU  - Rom WN
FAU - Takiguchi, Yuichi
AU  - Takiguchi Y
FAU - Jaishree, Jagirdai
AU  - Jaishree J
FAU - Nakata, Koh
AU  - Nakata K
FAU - Tatsumi, Koichiro
AU  - Tatsumi K
FAU - Mikata, Aatsuo
AU  - Mikata A
FAU - Kuriyama, Takatyuki
AU  - Kuriyama T
FAU - Weiden, Michael D
AU  - Weiden MD
LA  - eng
GR  - HL57879/HL/NHLBI NIH HHS/United States
GR  - M0RR00096/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Immunoglobulins)
RN  - 0 (Oligoclonal Bands)
RN  - 0 (Receptors, Antigen, T-Cell)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - HIV Infections/*complications
MH  - Humans
MH  - Immunoglobulins/genetics
MH  - Lung Diseases, Interstitial/*complications/*genetics/physiopathology
MH  - Lymphocytes, Tumor-Infiltrating/physiology
MH  - Lymphoma, AIDS-Related/*complications/*genetics/physiopathology
MH  - Lymphoproliferative Disorders/*complications/*genetics/physiopathology
MH  - Male
MH  - Middle Aged
MH  - Oligoclonal Bands
MH  - Polymerase Chain Reaction
MH  - Receptors, Antigen, T-Cell/genetics/physiology
MH  - Sequence Analysis
MH  - T-Lymphocytes/*physiology
EDAT- 2002/01/16 10:00
MHDA- 2002/02/22 10:01
CRDT- 2002/01/16 10:00
PHST- 2002/01/16 10:00 [pubmed]
PHST- 2002/02/22 10:01 [medline]
PHST- 2002/01/16 10:00 [entrez]
AID - 10.1164/ajrccm.165.2.2101141 [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2002 Jan 15;165(2):254-9. doi: 
      10.1164/ajrccm.165.2.2101141.