PMID- 11791924
OWN - NLM
STAT- MEDLINE
DCOM- 20020724
LR  - 20220316
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 23
IP  - 4
DP  - 2002 Feb
TI  - Characterization of porous collagen/hyaluronic acid scaffold modified by 
      1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking.
PG  - 1205-12
AB  - In order to develop a scaffolding material for tissue regeneration, porous 
      matrices containing collagen and hyaluronic acid were fabricated by freeze drying 
      at -20 degrees C, -70 degrees C or -196 degrees C. The fabricated porous 
      membranes were cross-linked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide 
      (EDC) in a range of 1-100 mM concentrations for enhancing mechanical stability of 
      the composite matrix. Scanning electron microscope (SEM) views of the matrices 
      demonstrated that the matrices obtained before cross-linking process had 
      interconnected pores with mean diameters of 40, 90 or 230 microm and porosity of 
      58-66% according to the freezing temperature, and also the porous structures 
      after cross-linking process were retained. The swelling test and IR spectroscopic 
      measurement of different cross-linked membranes were carried out as a measure of 
      the extent of cross-linking. The swelling behavior of cross-linked membranes 
      showed no significant differences as cross-linking degree increased. FT-IR 
      spectra showed the increase of the intensity of the absorbencies at amide bonds 
      (1655, 1546, 1458 cm(-1)) compared to that of CH bond (2930 cm(-1)). In enzymatic 
      degradation test, EDC treated membranes showed significant enhancement of the 
      resistance to collagenase activity in comparison with 0.625% glutaraldehyde 
      treated membranes. In cytotoxicity test using L929 fibroblastic cells, the 
      EDC-cross-linked membranes demonstrated no significant toxicity.
FAU - Park, Si-Nae
AU  - Park SN
AD  - Department of Medical Engineering, Yonsei University College of Medicine, Seoul, 
      South Korea.
FAU - Park, Jong-Chul
AU  - Park JC
FAU - Kim, Hea Ok
AU  - Kim HO
FAU - Song, Min Jung
AU  - Song MJ
FAU - Suh, Hwal
AU  - Suh H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 0 (Cross-Linking Reagents)
RN  - 73U5123RNT (1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide methiodide)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - 9007-34-5 (Collagen)
RN  - RJ5OZG6I4A (Ethyldimethylaminopropyl Carbodiimide)
SB  - IM
MH  - Animals
MH  - *Biocompatible Materials/chemistry/metabolism/toxicity
MH  - Biodegradation, Environmental
MH  - Cattle
MH  - Cell Line
MH  - *Collagen/chemistry/metabolism/toxicity
MH  - Cross-Linking Reagents
MH  - Ethyldimethylaminopropyl Carbodiimide/*analogs & derivatives
MH  - *Hyaluronic Acid/chemistry/metabolism/toxicity
MH  - Materials Testing
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Spectroscopy, Fourier Transform Infrared
MH  - *Tissue Engineering
EDAT- 2002/01/17 10:00
MHDA- 2002/07/26 10:01
CRDT- 2002/01/17 10:00
PHST- 2002/01/17 10:00 [pubmed]
PHST- 2002/07/26 10:01 [medline]
PHST- 2002/01/17 10:00 [entrez]
AID - S0142-9612(01)00235-6 [pii]
AID - 10.1016/s0142-9612(01)00235-6 [doi]
PST - ppublish
SO  - Biomaterials. 2002 Feb;23(4):1205-12. doi: 10.1016/s0142-9612(01)00235-6.