PMID- 11792124
OWN - NLM
STAT- MEDLINE
DCOM- 20020422
LR  - 20041117
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 16
IP  - 4
DP  - 2001 Nov 21
TI  - Gene expression profiles for Fc epsilon RI, cytokines and chemokines upon Fc 
      epsilon RI activation in human cultured mast cells derived from peripheral blood.
PG  - 143-52
AB  - Mast cells have been reported to release not only chemical mediators, but also 
      cytokines upon Fc epsilon receptor I(Fc epsilon RI) cross-linking. Recently, we 
      have established a culture system to derive chymase-rich human mast cells from 
      mononuclear cells in peripheral blood. However, the functional properties of 
      these mast cells have remained unrevealed. In this study, we examined the 
      functions of peripheral blood-derived human cultured mast cells (pHCMCs). pHCMCs 
      expressed functional Fc epsilon RI, and most of them contained tryptase. These 
      pHCMCs sensitized with immunoglobulin E (IgE) and interleukin 4 (IL-4) were 
      activated through cross-linking of Fc epsilon RI. The time-dependent mRNA 
      expression profiles of Fc epsilon RI subunits, cytokines and chemokines in the 
      sensitized pHCMCs upon Fc epsilon RI engagement were examined by reverse 
      transcriptase polymerase chain reaction (RT-PCR). mRNA for most of cytokines and 
      chemokines, which were observed in allergic inflammation, was detected in 
      activated pHCMCs. In addition, gene expression for monocyte chemoattractant 
      protein 3 (MCP-3) in human mast cells, and liver and activation-regulated 
      chemokine (LARC), thymus and activation-regulated chemokine (TARC) and 
      macrophage-derived chemokine (MDC) in mast cells was revealed for the first time 
      in our study. Fc epsilon RI-mediated cytokine and chemokine production at protein 
      level was evaluated using enzyme-linked immunosorbent assay (ELISA). These data 
      suggest that pHCMCs, which are capable of producing a variety of cytokines and 
      chemokines, can be a useful candidate for investigating roles of mast cells as a 
      conductor for allergic inflammation.
CI  - Copyright 2001 Academic Press.
FAU - Wakahara, S
AU  - Wakahara S
AD  - Molecular Biology Laboratory, Medicinal Research Laboratoiries, Taisho 
      Pharmaceutical Co., Ltd, Saitama, Japan.
FAU - Fujii, Y
AU  - Fujii Y
FAU - Nakao, T
AU  - Nakao T
FAU - Tsuritani, K
AU  - Tsuritani K
FAU - Hara, T
AU  - Hara T
FAU - Saito, H
AU  - Saito H
FAU - Ra, C
AU  - Ra C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, IgE)
RN  - 0 (Recombinant Proteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokines/biosynthesis/*genetics
MH  - Cytokines/biosynthesis/*genetics
MH  - Gene Expression Profiling
MH  - Humans
MH  - Hypersensitivity/etiology/immunology
MH  - Immunoglobulin E/pharmacology
MH  - Inflammation/etiology/immunology
MH  - Interleukin-4/pharmacology
MH  - Mast Cells/drug effects/*immunology/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, IgE/*genetics/*metabolism
MH  - Recombinant Proteins/pharmacology
EDAT- 2002/01/17 10:00
MHDA- 2002/04/23 10:01
CRDT- 2002/01/17 10:00
PHST- 2002/01/17 10:00 [pubmed]
PHST- 2002/04/23 10:01 [medline]
PHST- 2002/01/17 10:00 [entrez]
AID - S1043466601909585 [pii]
AID - 10.1006/cyto.2001.0958 [doi]
PST - ppublish
SO  - Cytokine. 2001 Nov 21;16(4):143-52. doi: 10.1006/cyto.2001.0958.