PMID- 11792488
OWN - NLM
STAT- MEDLINE
DCOM- 20020701
LR  - 20190901
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 85
IP  - 1
DP  - 2002 Feb 26
TI  - Porcine reproductive and respiratory syndrome virus (PRRSV) infection status in 
      pigs naturally affected with post-weaning multisystemic wasting syndrome (PMWS) 
      in Spain.
PG  - 23-30
AB  - The purpose of the study reported here was to determine the prevalence of porcine 
      reproductive and respiratory syndrome virus (PRRSV) in pigs affected with 
      post-weaning multisystemic wasting syndrome (PMWS), a disease believed to be 
      caused by porcine circovirus type 2 (PCV2). From May 1997 to February 2000, PMWS 
      was diagnosed in 277 pigs (from 120 farms) submitted to the Veterinary Pathology 
      Diagnostic Service, Veterinary School of Barcelona, Spain. In each case, the PMWS 
      diagnosis was based on clinical history and the detection, by in situ 
      hybridization, of nucleic acid of PCV2 in characteristic histologic lesions. 
      Antigens for PRRSV were detected by immunohistochemistry in tissues of 66 (23.8%) 
      of the same 277 pigs. Sera, which were available for 93 of the 277 pigs, were 
      tested for PRRSV by a multiplex reverse transcription-polymerase chain reaction 
      (RT-PCR). A total of 33 of these sera were RT-PCR positive, three for a North 
      American strain(s) of PRRSV. In addition, 76 of the 93 sera were tested for 
      antibodies to PCV2 (indirect immunoperoxidase) and PRRSV (enzyme-linked 
      immunoassay). Antibodies for PCV2 and PRRSV were detected, respectively, in 56 
      (73.9%) and 43 (56.6%) of the 76 sera. Collectively, these results suggest that 
      while infection with PRRSV may be common, it is not an essential component of 
      PMWS.
FAU - Segales, Joaquim
AU  - Segales J
AD  - Centre de Recerca en Sanitat Animal (CReSA), Departament de Sanitat i d'Anatomia 
      Animals, Facultat de Veterinaria, Universitat Autonoma de Barcelona, E-08193 
      Bellaterra, Barcelona, Spain. joaquim.segales@uab.es
FAU - Calsamiglia, Maria
AU  - Calsamiglia M
FAU - Rosell, Carles
AU  - Rosell C
FAU - Soler, Merce
AU  - Soler M
FAU - Maldonado, Jaime
AU  - Maldonado J
FAU - Martin, Marga
AU  - Martin M
FAU - Domingo, Mariano
AU  - Domingo M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antigens, Viral/blood
MH  - Circoviridae Infections/complications/epidemiology/*veterinary
MH  - Circovirus
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Immunohistochemistry/veterinary
MH  - Lung/pathology
MH  - Porcine Reproductive and Respiratory Syndrome/*epidemiology
MH  - Porcine respiratory and reproductive syndrome 
      virus/genetics/immunology/*isolation & purification
MH  - Prevalence
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Spain/epidemiology
MH  - Swine
MH  - Swine Diseases/*epidemiology/virology
MH  - Wasting Syndrome/complications/*veterinary/virology
MH  - Weaning
EDAT- 2002/01/17 10:00
MHDA- 2002/07/02 10:01
CRDT- 2002/01/17 10:00
PHST- 2002/01/17 10:00 [pubmed]
PHST- 2002/07/02 10:01 [medline]
PHST- 2002/01/17 10:00 [entrez]
AID - S0378113501004746 [pii]
AID - 10.1016/s0378-1135(01)00474-6 [doi]
PST - ppublish
SO  - Vet Microbiol. 2002 Feb 26;85(1):23-30. doi: 10.1016/s0378-1135(01)00474-6.