PMID- 11792561
OWN - NLM
STAT- MEDLINE
DCOM- 20020429
LR  - 20220321
IS  - 8756-3282 (Print)
IS  - 1873-2763 (Linking)
VI  - 30
IP  - 1
DP  - 2002 Jan
TI  - Macrophage cell lines produce osteoinductive signals that include bone 
      morphogenetic protein-2.
PG  - 26-31
AB  - Bone wound healing requires osteoinductive signals that are attributed to (the) 
      bone morphogenetic proteins (BMPs). The cellular origin of such osteoinductive 
      signals has only been partially elucidated. Because of the central role of the 
      macrophage in cutaneous wound healing, we hypothesized that the macrophage could 
      play a similar role in osseous healing. It was the aim of the present 
      investigation to examine the possible expression of BMP by the macrophage, and to 
      evaluate the contribution of macrophage products to an early step of bone 
      formation modeled in an in vitro culture system. The synthesis of BMP-2 and BMP-6 
      by cultured human and murine macrophages was evaluated by reverse 
      transcription-polymerase chain reaction (RT-PCR). When human mesenchymal stem 
      cells (hMSCs) were grown in conditioned media from J774A.1 cells, alkaline 
      phosphatase expression increased. This induction was blocked by anti-BMP-2 
      antibody and by anti-transforming growth factor-beta1 (TGF-beta1) antibody. 
      Modeling of the macrophage expression of osteoinductive signals by potential 
      physiological situations was evaluated by treatments with lipopolysaccharide 
      (LPS) or macrophage chemotactic peptide-1 (MCP-1). Macrophage BMP-2 expression 
      was reduced by proinflammatory LPS stimulation (which was confirmed to induce 
      release of the proinflammatory cytokine, TNF-alpha), and conditioned media from 
      LPS-treated macrophages had no ability to increase alkaline phosphatase activity 
      in hMSCs. This first study of macrophage BMP-2 expression indicates that the 
      macrophage is capable of physiological regulation consistent with a key role in 
      osteoinduction for osseous wound healing.
FAU - Champagne, C M
AU  - Champagne CM
AD  - Dental Research Center, University of North Carolina School of Dentistry, Chapel 
      Hill, NC 27599-7450, USA.
FAU - Takebe, J
AU  - Takebe J
FAU - Offenbacher, S
AU  - Offenbacher S
FAU - Cooper, L F
AU  - Cooper LF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bone
JT  - Bone
JID - 8504048
RN  - 0 (BMP2 protein, human)
RN  - 0 (BMP6 protein, human)
RN  - 0 (Bmp2 protein, mouse)
RN  - 0 (Bmp6 protein, mouse)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Protein 6)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/biosynthesis
MH  - Animals
MH  - Bone Morphogenetic Protein 2
MH  - Bone Morphogenetic Protein 6
MH  - Bone Morphogenetic Proteins/*biosynthesis/genetics
MH  - Cell Differentiation
MH  - Cell Line
MH  - Culture Media, Conditioned
MH  - Fracture Healing/genetics/*physiology
MH  - Gene Expression
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/*physiology
MH  - Mice
MH  - Models, Biological
MH  - Osteoblasts/cytology
MH  - Osteogenesis/genetics/physiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Signal Transduction
MH  - Stem Cells/cytology
MH  - *Transforming Growth Factor beta
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 2002/01/17 10:00
MHDA- 2002/05/01 10:01
CRDT- 2002/01/17 10:00
PHST- 2002/01/17 10:00 [pubmed]
PHST- 2002/05/01 10:01 [medline]
PHST- 2002/01/17 10:00 [entrez]
AID - S875632820100638X [pii]
AID - 10.1016/s8756-3282(01)00638-x [doi]
PST - ppublish
SO  - Bone. 2002 Jan;30(1):26-31. doi: 10.1016/s8756-3282(01)00638-x.