PMID- 11793393
OWN - NLM
STAT- MEDLINE
DCOM- 20020423
LR  - 20190901
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 66
IP  - 3
DP  - 2002 Mar
TI  - HHV-6 A- or B-specific P41 antigens do not reveal virus variant-specific IgG or 
      IgM responses in human serum.
PG  - 394-9
AB  - The etiology of multiple sclerosis (MS) remains unknown, but there are 
      indications of a role of human herpesvirus 6 (HHV-6), especially variant A, in 
      the pathogenesis. Higher serum antibody reactivity against an HHV-6 early 
      protein, p41, has been found in MS cases than in controls. The antigen, however, 
      was purified from infected cells with a monoclonal antibody also reactive with a 
      protein (p38) likely to be of cellular origin. To avoid serological 
      crossreactivity with the cellular protein, recombinant p41 proteins from HHV-6A 
      strain GS and HHV-6B strain Z29 were expressed as glutathione-S-transferase 
      fusion proteins (p41-GST), and used as antigens in an enzyme-linked immunosorbent 
      assay (ELISA). p41 variant specific monoclonal antibodies reacted strongly with 
      the respective recombinant proteins. Serum IgM and IgG reactivities with the 
      recombinant p41 antigens were analysed in patients with manifest MS, patients 
      with optic neuritis, patients with other neurological diseases, and in one group 
      of healthy controls. All sera were HHV-6 IgG seropositive by immunofluorescence. 
      The serum IgM or IgG reactivities against the recombinant p41 antigens did not 
      differ significantly between the groups, and the reactivities against the variant 
      A and B antigens were identical. In many samples, the reactivity was very low. 
      The results indicate that p41 is not an optimal target for HHV-6 serology 
      studies, and that the data obtained with the p41 antigen prepared from infected 
      cells (possibly including also p38) should be interpreted with caution.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Xu, Yunhe
AU  - Xu Y
AD  - Department of Virology, Swedish Institute for Infectious Disease Control, Solna, 
      Sweden. Yunhe.Xu@cgb.ki.se
FAU - Linde, Annika
AU  - Linde A
FAU - Fredrikson, Sten
AU  - Fredrikson S
FAU - Dahl, Helena
AU  - Dahl H
FAU - Winberg, Gosta
AU  - Winberg G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Proteins)
RN  - 138415-18-6 (p41 protein, Human herpesvirus 6)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Antibodies, Viral/blood/*immunology
MH  - Antigenic Variation/*immunology
MH  - Antigens, Viral/*immunology
MH  - Competitive Bidding
MH  - DNA-Binding Proteins/*immunology
MH  - Herpesvirus 6, Human/*immunology/isolation & purification
MH  - Humans
MH  - Immunoglobulin G/blood/immunology
MH  - Immunoglobulin M/blood/immunology
MH  - Multiple Sclerosis/blood/immunology/virology
MH  - Optic Neuritis/blood/immunology/virology
MH  - Recombinant Fusion Proteins/immunology
MH  - Roseolovirus Infections/blood/immunology/*virology
MH  - Viral Proteins/*immunology
EDAT- 2002/01/17 10:00
MHDA- 2002/04/24 10:01
CRDT- 2002/01/17 10:00
PHST- 2002/01/17 10:00 [pubmed]
PHST- 2002/04/24 10:01 [medline]
PHST- 2002/01/17 10:00 [entrez]
AID - 10.1002/jmv.2158 [pii]
AID - 10.1002/jmv.2158 [doi]
PST - ppublish
SO  - J Med Virol. 2002 Mar;66(3):394-9. doi: 10.1002/jmv.2158.