PMID- 11793398
OWN - NLM
STAT- MEDLINE
DCOM- 20020423
LR  - 20190901
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 66
IP  - 3
DP  - 2002 Mar
TI  - Frequent detection of the replicative form of TT virus DNA in peripheral blood 
      mononuclear cells and bone marrow cells in cancer patients.
PG  - 428-34
AB  - The TT virus (TTV), a member of a family of human viruses related to the 
      circoviridae viruses, was associated initially with acute and chronic liver 
      diseases. TTV consists of a single-stranded, circular DNA genome of 3.8 kilobases 
      (kb) and at least three open reading frames (ORFs). The objective of the present 
      study was to determine whether or not TTV replicated in peripheral blood 
      mononuclear cells (PBMCs) and bone marrow cells (BMCs). DNA was extracted from 
      the PBMCs or BMCs of 153 cancer patients and from the PBMCs of 50 healthy blood 
      donors (the controls). By using a single round of polymerase chain reaction 
      (PCR), TTV was detected in 98.6% (141 of 143) of the PBMCs and in 90% (9 of 10) 
      of the BMCs from cancer patients. TTV DNA was detected in significantly fewer 
      control subjects at 86% (43 of 50; P < 0.05). Strand-specific PCR (SSPCR) 
      targeting the ORF2 of the common genotypes of TTV was developed specifically to 
      detect TTV positive or negative strand DNA and to examine TTV replication. TTV 
      positive strand DNA, which may be an intermediate of viral replication, was 
      detected in 55.3% (78 of 141) of the TTV-infected PBMCs of the cancer patients 
      and in 7% (3 of 43) of the controls (P < 0.001). The replicative form of TTV was 
      also detectable in 55.6% (5 of 9) of the TTV-infected BMCs. The existence of 
      double-strand (positive and negative strands) TTV DNA in PBMCs and BMCs of the 
      cancer patients was also supported by the finding that TTV DNA extracted from 
      these cells was resistant to S1 nuclease. Using in situ hybridization, TTV DNA 
      was also demonstrated to be present in the nucleus of PBMCs. It is concluded that 
      replicative intermediate forms of TTV DNA are present in both PBMCs and BMCs, 
      indicating that blood cells may be a site of TTV replication.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Zhong, Sheng
AU  - Zhong S
AD  - Department of Clinical Oncology, Sir Y.K. Pao Centre for Cancer, Chinese 
      University of Hong Kong, Prince of Wales Hospital, Shatin, NT, Hong Kong.
FAU - Yeo, Winnie
AU  - Yeo W
FAU - Tang, Mandy
AU  - Tang M
FAU - Liu, Cuiling
AU  - Liu C
FAU - Lin, Xiao-rong
AU  - Lin XR
FAU - Ho, Wing M
AU  - Ho WM
FAU - Hui, Pun
AU  - Hui P
FAU - Johnson, Philip J
AU  - Johnson PJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Bone Marrow Cells/*virology
MH  - Breast Neoplasms/blood/complications/virology
MH  - Carcinoma, Hepatocellular/blood/complications/virology
MH  - Colonic Neoplasms/blood/complications/virology
MH  - DNA Virus Infections/blood/complications/*virology
MH  - DNA, Viral/blood
MH  - Female
MH  - Humans
MH  - In Situ Hybridization/methods
MH  - Leukocytes, Mononuclear/*virology
MH  - Liver Neoplasms/blood/complications/virology
MH  - Lymphoma, Non-Hodgkin/blood/complications/virology
MH  - Multiple Myeloma/blood/complications/virology
MH  - Nasopharyngeal Neoplasms/blood/complications/virology
MH  - Neoplasms/blood/complications/*virology
MH  - Torque teno virus/genetics/*isolation & purification
MH  - *Virus Replication
EDAT- 2002/01/17 10:00
MHDA- 2002/04/24 10:01
CRDT- 2002/01/17 10:00
PHST- 2002/01/17 10:00 [pubmed]
PHST- 2002/04/24 10:01 [medline]
PHST- 2002/01/17 10:00 [entrez]
AID - 10.1002/jmv.2163 [pii]
AID - 10.1002/jmv.2163 [doi]
PST - ppublish
SO  - J Med Virol. 2002 Mar;66(3):428-34. doi: 10.1002/jmv.2163.