PMID- 11793416
OWN - NLM
STAT- MEDLINE
DCOM- 20020213
LR  - 20101118
IS  - 0275-2565 (Print)
IS  - 0275-2565 (Linking)
VI  - 56
IP  - 2
DP  - 2002 Feb
TI  - Comparison of chorionic gonadotropin expression in human and macaque (Macaca 
      fascicularis) trophoblasts.
PG  - 89-97
AB  - We have designed novel DNA primers that allow us to detect the expression of the 
      subunits of chorionic gonadotropin (CG) from a variety of species of the order 
      Primates. Using these primers, reverse transcriptase-polymerase chain reaction 
      (RT-PCR), and standard cloning techniques, we detected the expression of a single 
      gene for the common glycoprotein hormone (GPH) alpha-subunit and at least two 
      genes for the CG beta-subunit in trophoblasts of Macaca fascicularis (cynomolgous 
      macaque (cm)) at gestational day (GD) = 26 (+/- 2d). No cmCG expression was 
      detected at GD = 35-40. When sequences of cmGPH-alpha and cmCG-beta genes were 
      compared to the corresponding genes of other primates, we found that the 
      alpha-subunit of M. fascicularis was highly conserved compared to other primate 
      species. However, cmCG beta-subunits appeared to be less conserved, residing 
      between those of human CG-beta and baboon CG-beta when analyzed phylogenetically. 
      Of particular interest was a three amino acid stretch in one of the expressed 
      cmCG-beta genes that is distinct from all other primates studied. Our findings 
      imply that not only does the expression of multiple CG beta-subunit genes appear 
      to be common to Old World monkeys, but that the presented methodology will 
      greatly facilitate our ability to understand primate evolution.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Wilken, Jason A
AU  - Wilken JA
AD  - Eppley Institute for Research in Cancer and Allied Diseases, 986805 University of 
      Nebraska Medical Center, Omaha, NE 68198-6805, USA.
FAU - Matsumoto, Kikuko
AU  - Matsumoto K
FAU - Laughlin, Lisa S
AU  - Laughlin LS
FAU - Lasley, Bill L
AU  - Lasley BL
FAU - Bedows, Elliott
AU  - Bedows E
LA  - eng
GR  - CA 09746/CA/NCI NIH HHS/United States
GR  - CA 32949/CA/NCI NIH HHS/United States
GR  - ES 04699/ES/NIEHS NIH HHS/United States
GR  - ES 06198/ES/NIEHS NIH HHS/United States
GR  - P30 CA 36727/CA/NCI NIH HHS/United States
GR  - RR 00169/RR/NCRR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Primatol
JT  - American journal of primatology
JID - 8108949
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Chorionic Gonadotropin, beta Subunit, Human)
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Biological Evolution
MH  - Chorionic Gonadotropin/*biosynthesis/genetics
MH  - Chorionic Gonadotropin, beta Subunit, Human/*biosynthesis/genetics
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Female
MH  - *Gene Expression Regulation
MH  - Gestational Age
MH  - Humans
MH  - Macaca fascicularis/genetics/*physiology
MH  - Molecular Sequence Data
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trophoblasts
EDAT- 2002/01/17 10:00
MHDA- 2002/02/14 10:01
CRDT- 2002/01/17 10:00
PHST- 2002/01/17 10:00 [pubmed]
PHST- 2002/02/14 10:01 [medline]
PHST- 2002/01/17 10:00 [entrez]
AID - 10.1002/ajp.1066 [pii]
AID - 10.1002/ajp.1066 [doi]
PST - ppublish
SO  - Am J Primatol. 2002 Feb;56(2):89-97. doi: 10.1002/ajp.1066.