PMID- 11793439
OWN - NLM
STAT- MEDLINE
DCOM- 20020131
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 33
IP  - 2
DP  - 2002 Feb
TI  - Differentially amplified chromosome 12 sequences in low- and high-grade 
      osteosarcoma.
PG  - 133-40
AB  - Most osteosarcomas are highly aggressive malignancies characterized by a complex 
      pattern of chromosome abnormalities. However, a subgroup of low-grade, parosteal 
      tumors exhibits a relatively simple aberration pattern dominated by ring 
      chromosomes carrying amplified material from chromosome 12. To assess whether 
      sequences from this chromosome were differentially amplified in low- and 
      high-grade osteosarcomas, copy numbers of the CCND2, ETV6, KRAS2, and D12S85 
      regions in 12p and the MDM2 region in 12q were evaluated by interphase or 
      metaphase fluorescence in situ hybridization (FISH) in 24 osteosarcomas. 
      Amplification of MDM2 was detected in all five low-grade and four high-grade 
      osteosarcomas, all of which showed ring chromosomes. An overrepresentation of 12p 
      sequences was found in 1/5 low-grade and in 9/19 high-grade tumors. Multicolor 
      single-copy FISH analysis of metaphase cells from six high-grade tumors showed 
      that extra 12p material either occurred together with MDM2 in ring chromosomes or 
      was scattered over the genome as a result of complex structural rearrangements. 
      Most tumors (8/10) not containing amplification of the assessed chromosome 12 
      loci exhibited a nondiploid pattern at evaluation with probes for centromeric 
      alpha satellite sequences. These findings indicate that gain of sequences from 
      the short arm of chromosome 12 could be a possible genetic pathway in the 
      development of aggressive osteosarcoma.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Gisselsson, David
AU  - Gisselsson D
AD  - Department of Clinical Genetics, University Hospital, Lund, Sweden. 
      david.gisselsson@klingen.lu.se
FAU - Palsson, Eva
AU  - Palsson E
FAU - Hoglund, Mattias
AU  - Hoglund M
FAU - Domanski, Henryk
AU  - Domanski H
FAU - Mertens, Fredrik
AU  - Mertens F
FAU - Pandis, Nikos
AU  - Pandis N
FAU - Sciot, Raf
AU  - Sciot R
FAU - Dal Cin, Paola
AU  - Dal Cin P
FAU - Bridge, Julia A
AU  - Bridge JA
FAU - Mandahl, Nils
AU  - Mandahl N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Base Sequence
MH  - Bone Neoplasms/*genetics/pathology
MH  - Child
MH  - Chromosome Banding
MH  - Chromosomes, Human, Pair 12/*genetics
MH  - Female
MH  - Gene Amplification/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Karyotyping
MH  - Male
MH  - Metaphase/genetics
MH  - Middle Aged
MH  - Osteosarcoma/*genetics/pathology
MH  - Tumor Cells, Cultured
EDAT- 2002/01/17 10:00
MHDA- 2002/02/01 10:01
CRDT- 2002/01/17 10:00
PHST- 2002/01/17 10:00 [pubmed]
PHST- 2002/02/01 10:01 [medline]
PHST- 2002/01/17 10:00 [entrez]
AID - 10.1002/gcc.1219 [pii]
AID - 10.1002/gcc.1219 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2002 Feb;33(2):133-40. doi: 10.1002/gcc.1219.