PMID- 11793448
OWN - NLM
STAT- MEDLINE
DCOM- 20020131
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 33
IP  - 2
DP  - 2002 Feb
TI  - Differentiation of follicular from mucosa-associated lymphoid tissue lymphoma by 
      detection of t(14;18) on single-cell preparations and paraffin-embedded sections.
PG  - 213-6
AB  - A 57-year-old woman was referred to the Kyoto Prefectural University of Medicine 
      because of multiple polypoid lesions in the duodenum. On the basis of the 
      histopathologic findings, mucosa-associated lymphoid tissue lymphoma had been 
      diagnosed. The polypoid lesions did not show any improvement in spite of 
      antimicrobial therapy for elimination of Helicobacter pylori. Because the disease 
      remained stable during the clinical course, no other specific treatment was 
      administered. We performed fluorescence in situ hybridization (FISH) analysis on 
      a single-cell preparation obtained from the duodenal lesions, to assess the 
      specific chromosome translocation. We identified BCL2/IGH fusion at a frequency 
      of 83%. Two-color FISH was also performed on paraffin-embedded tissue sections, 
      which demonstrated BCL2/IGH fusion-positive cells in neoplastic follicles. These 
      findings, together with the CD10+ immunophenotyping of tumor cells, led to a 
      diagnosis of primary follicular lymphoma of the duodenum. Interphase FISH with 
      the IGH gene and oncogene probes is a rapid and powerful tool for assessing 
      genomic changes in gastrointestinal lymphoma on single-cell preparations and 
      tissue sections. This technique is particularly useful in view of the 
      increasingly small core biopsy samples and needle aspirations obtained for 
      diagnostic purposes.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Nomura, Kenichi
AU  - Nomura K
AD  - Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, 
      Kawaramachi-Hirokouji, Kyoto, Japan. nomuken@sun.kpu-m.ac.jp
FAU - Sekoguchi, Satoru
AU  - Sekoguchi S
FAU - Ueda, Kyoji
AU  - Ueda K
FAU - Nakao, Mitsushige
AU  - Nakao M
FAU - Akano, Yumiko
AU  - Akano Y
FAU - Fujita, Yasuko
AU  - Fujita Y
FAU - Yamashita, Yasuhide
AU  - Yamashita Y
FAU - Horiike, Shigeo
AU  - Horiike S
FAU - Nishida, Kazuhiro
AU  - Nishida K
FAU - Nakamura, Shigeo
AU  - Nakamura S
FAU - Taniwaki, Masafumi
AU  - Taniwaki M
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Chromosomes, Human, Pair 14/*genetics
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell, Marginal Zone/*diagnosis/*genetics/pathology
MH  - Lymphoma, Follicular/*diagnosis/*genetics/pathology
MH  - Middle Aged
MH  - Paraffin Embedding/methods
MH  - Translocation, Genetic/*genetics
EDAT- 2002/01/17 10:00
MHDA- 2002/02/01 10:01
CRDT- 2002/01/17 10:00
PHST- 2002/01/17 10:00 [pubmed]
PHST- 2002/02/01 10:01 [medline]
PHST- 2002/01/17 10:00 [entrez]
AID - 10.1002/gcc.10013 [pii]
AID - 10.1002/gcc.10013 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2002 Feb;33(2):213-6. doi: 10.1002/gcc.10013.