PMID- 11795297
OWN - NLM
STAT- MEDLINE
DCOM- 20020201
LR  - 20190616
IS  - 0077-8923 (Print)
IS  - 0077-8923 (Linking)
VI  - 947
DP  - 2001 Dec
TI  - Differential signaling for MCP-1-dependent integrin activation and chemotaxis.
PG  - 387-9
AB  - Transmigration of monocytes to the subendothelial space is the initial step in 
      atherosclerotic plaque formation and inflammation. Integrin activation and 
      chemotaxis are two important functions in monocyte transmigration. To delineate 
      the signaling cascades leading to integrin activation and chemotaxis by monocyte 
      chemoattractant protein-1 (MCP-1), we investigated the roles of MAPK in THP-1 
      cells, a monocytic cell line. MCP-1 stimulated beta1 integrin-dependent, but not 
      beta2 integrin-dependent cell adhesion in a time-dependent manner. MCP-1-mediated 
      cell adhesion was inhibited by a MEK inhibitor, but not by a p38-MAPK inhibitor. 
      By contrast, MCP-1-mediated chemotaxis was inhibited by the p38-MAPK inhibitor, 
      but not by the MEK inhibitor. These data indicate that ERK is responsible for 
      integrin activation and that p38-MAPK is responsible for chemotaxis mediated by 
      MCP-1. This study demonstrates that two distinct MAPKs regulate two dependent 
      signaling cascades, leading to integrin activation and chemotaxis induced by 
      MCP-1 in THP-1 cells.
FAU - Ashida, N
AU  - Ashida N
AD  - Department of Geriatric Medicine, Kyoto University Graduate School of Medicine, 
      Japan. ash@kuhp.kyoto.u.ac.jp
FAU - Arai, H
AU  - Arai H
FAU - Yamasaki, M
AU  - Yamasaki M
FAU - Kita, T
AU  - Kita T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Fibronectins)
RN  - 0 (Flavonoids)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/*pharmacology
MH  - *Chemotaxis, Leukocyte/drug effects/*physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Fibronectins/metabolism
MH  - Flavonoids/pharmacology
MH  - Humans
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors
MH  - Monocytes/drug effects/physiology
MH  - Signal Transduction/*physiology
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2002/01/25 10:00
MHDA- 2002/02/02 10:01
CRDT- 2002/01/25 10:00
PHST- 2002/01/25 10:00 [pubmed]
PHST- 2002/02/02 10:01 [medline]
PHST- 2002/01/25 10:00 [entrez]
AID - 10.1111/j.1749-6632.2001.tb03969.x [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2001 Dec;947:387-9. doi: 10.1111/j.1749-6632.2001.tb03969.x.