PMID- 11795986
OWN - NLM
STAT- MEDLINE
DCOM- 20020311
LR  - 20161018
IS  - 1063-4584 (Print)
IS  - 1063-4584 (Linking)
VI  - 9
IP  - 8
DP  - 2001 Nov
TI  - A single injection of fibronectin fragments into rabbit knee joints enhances 
      catabolism in the articular cartilage followed by reparative responses but also 
      induces systemic effects in the non-injected knee joints.
PG  - 673-83
AB  - OBJECTIVE: To investigate effects on cartilage metabolism and degeneration of 
      injection of fibronectin fragments (Fn-fs) into rabbit knee joints. DESIGN: The 
      knees of adolescent New Zealand white rabbits were intraarticularly injected with 
      rabbit Fn-fs. Cartilage sections from both injected and non-injected joints were 
      treated with Safranin-O, with antibodies to the VDIPEN and NITEGE neoepitopes of 
      degraded aggrecan and to matrix metalloproteinase-3 (MMP-3). Proteoglycan (PG) 
      content of cartilage was measured by a dimethylmethylene blue assay of papain 
      digests. PG synthesis rates were measured by(35)S-sodium sulfate incorporation 
      into explanted cartilage. RESULTS: In the injected joint cartilage, the Fn-fs 
      bound cells in the upper superficial zone maximally between 6 and 24 h. By day 2, 
      MMP-3 protein was enhanced and cartilage PG content and PG synthesis rates were 
      reduced 40% and 70%, respectively. MMP-3 epitope and VDIPEN and NITEGE 
      neoepitopes were also enhanced. The PG content then increased to supernormal 
      levels from days 14 to 35 and then declined to normal levels by day 70, as did PG 
      synthesis rates. In the non-injected joint cartilage, Fn-fs were not detected. 
      Although MMP-3 expression was enhanced between days 2 and 21 as well as VDIPEN 
      neoepitope, the PG content was never reduced but rather enhanced to supernormal 
      levels from days 21 to 35. This was associated with enhanced PG synthesis by day 
      7, which decreased to control levels by day 70. CONCLUSIONS: In this cartilage 
      degeneration model, loss of cartilage PG is followed by supernormal anabolic 
      responses that facilitate PG restoration. Further, the damage causes a systemic 
      effect of enhanced PG synthesis and content in the non-injected joint cartilage.
CI  - Copyright 2001 OsteoArthritis Research Society International.
FAU - Homandberg, G A
AU  - Homandberg GA
AD  - Department of Biochemistry, Section of Rheumatology, Rush Medical College at 
      Rush-Presbyterian-St Luke's Medical Center, 1653 West Congress Parkway, Chicago, 
      Illinois 60612-3864, USA.
FAU - Kang, Y
AU  - Kang Y
FAU - Zhang, J
AU  - Zhang J
FAU - Cole, A A
AU  - Cole AA
FAU - Williams, J M
AU  - Williams JM
LA  - eng
GR  - AR39293-03/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 0 (Epitopes)
RN  - 0 (Fibronectins)
RN  - 0 (Oligopeptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (Proteoglycans)
RN  - 0 (peptide VDIPEN)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.99.- (aggrecanase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Biotinylation
MH  - Blotting, Western
MH  - Cartilage, Articular/*metabolism
MH  - Densitometry
MH  - Dose-Response Relationship, Drug
MH  - Endopeptidases/metabolism
MH  - Epitopes
MH  - Fibronectins/administration & dosage/*pharmacology
MH  - Injections, Intra-Articular
MH  - Knee Joint
MH  - Male
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Oligopeptides/metabolism
MH  - Peptide Fragments/metabolism
MH  - Proteoglycans/metabolism
MH  - Rabbits
MH  - Staining and Labeling
EDAT- 2002/02/14 10:00
MHDA- 2002/03/12 10:01
CRDT- 2002/02/14 10:00
PHST- 2002/02/14 10:00 [pubmed]
PHST- 2002/03/12 10:01 [medline]
PHST- 2002/02/14 10:00 [entrez]
AID - S1063-4584(01)90419-4 [pii]
AID - 10.1053/joca.2001.0419 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 2001 Nov;9(8):673-83. doi: 10.1053/joca.2001.0419.