PMID- 11797219
OWN - NLM
STAT- MEDLINE
DCOM- 20020226
LR  - 20201208
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 17
IP  - 5
DP  - 2001 Sep
TI  - [Expression and purification of murine interleukin 18 in Escherichia coli and its 
      antitumor effects].
PG  - 548-52
AB  - Total RNA was extracted from murine hepatocytes, and the cDNA of interleukin 
      18(IL-18) was amplified by RT-PCR. The cDNA was introduced into the expression 
      vector pJW2 and sequenced. Under heat induction, the recombinant murine 
      IL-18(rmIL-18) was expressed in inclusion bodies in E. coli with the yield 
      accounting for 18% of total bacteria proteins. The inclusion bodies were 
      dissolved with 5 mol/L urea, and rmIL-18 was purified using Sephadex G-100 column 
      chromatography. In the presence of 0.5 mg/L Con A, the purified rmIL-18 showed 
      dose-dependent IFN-gamma-inducing activity in murine splenocytes. The purified 
      rmIL-18 exhibited significant antitumor effects in Kunming mice challenged 
      intraperitoneally (i.p.) with H22 hepatocarcinoma when administered 10 micrograms 
      rmIL-18 i.p. on days 1, 4 after challenge, and the mice survived resisted the 
      rechallenged with H22 cells.
FAU - Zhou, H
AU  - Zhou H
AD  - Research Center for Biochemistry and Molecular Biology, Xuzhou Medical College, 
      Xuzhou 221002, China.
FAU - Zhao, H R
AU  - Zhao HR
FAU - Pei, D S
AU  - Pei DS
FAU - Lu, L
AU  - Lu L
FAU - Hu, S Q
AU  - Hu SQ
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (Antineoplastic Agents)
RN  - 0 (DNA, Complementary)
RN  - 0 (Interleukin-18)
RN  - 0 (Recombinant Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*pharmacology
MH  - Chromatography, Gel/methods
MH  - DNA, Complementary/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Escherichia coli/*genetics
MH  - Gene Expression
MH  - Interferon-gamma/metabolism
MH  - Interleukin-18/genetics/isolation & purification/*pharmacology
MH  - Liver Neoplasms, Experimental/immunology/pathology/prevention & control
MH  - Mice
MH  - Neoplasm Transplantation
MH  - Recombinant Proteins/immunology/isolation & purification/pharmacology
MH  - Spleen/cytology/drug effects/metabolism
MH  - Time Factors
MH  - Tumor Cells, Cultured
EDAT- 2002/01/19 10:00
MHDA- 2002/02/28 10:01
CRDT- 2002/01/19 10:00
PHST- 2002/01/19 10:00 [pubmed]
PHST- 2002/02/28 10:01 [medline]
PHST- 2002/01/19 10:00 [entrez]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2001 Sep;17(5):548-52.