PMID- 11797691
OWN - NLM
STAT- MEDLINE
DCOM- 20020201
LR  - 20190616
IS  - 0077-8923 (Print)
IS  - 0077-8923 (Linking)
VI  - 944
DP  - 2001 Nov
TI  - The influence of extracellular matrix on the generation of vascularized, 
      engineered, transplantable tissue.
PG  - 429-42
AB  - In a recently described model for tissue engineering, an arteriovenous loop 
      comprising the femoral artery and vein with interposed vein graft is fabricated 
      in the groin of an adult male rat, placed inside a polycarbonate chamber, and 
      incubated subcutaneously. New vascularized granulation tissue will generate on 
      this loop for up to 12 weeks. In the study described in this paper three 
      different extracellular matrices were investigated for their ability to 
      accelerate the amount of tissue generated compared with a no-matrix control. 
      Poly-D,L-lactic-co-glycolic acid (PLGA) produced the maximal weight of new tissue 
      and vascularization and this peaked at two weeks, but regressed by four weeks. 
      Matrigel was next best. It peaked at four weeks but by eight weeks it also had 
      regressed. Fibrin (20 and 80 mg/ml), by contrast, did not integrate with the 
      generating vascularized tissue and produced less weight and volume of tissue than 
      controls without matrix. The limiting factors to growth appear to be the chamber 
      size and the capacity of the neotissue to integrate with the matrix. Once the 
      sides of the chamber are reached or tissue fails to integrate, encapsulation and 
      regression follow. The intrinsic position of the blood supply within the 
      neotissue has many advantages for tissue and organ engineering, such as ability 
      to seed the construct with stem cells and microsurgically transfer new tissue to 
      another site within the individual. In conclusion, this study has found that PLGA 
      and Matrigel are the best matrices for the rapid growth of new vascularized 
      tissue suitable for replantation or transplantation.
FAU - Cassell, O C
AU  - Cassell OC
AD  - Bernard O'Brien Institute of Microsurgery, University of Melbourne, St. Vincent's 
      Hospital, Fitzroy, Victoria, Australia.
FAU - Morrison, W A
AU  - Morrison WA
FAU - Messina, A
AU  - Messina A
FAU - Penington, A J
AU  - Penington AJ
FAU - Thompson, E W
AU  - Thompson EW
FAU - Stevens, G W
AU  - Stevens GW
FAU - Perera, J M
AU  - Perera JM
FAU - Kleinman, H K
AU  - Kleinman HK
FAU - Hurley, J V
AU  - Hurley JV
FAU - Romeo, R
AU  - Romeo R
FAU - Knight, K R
AU  - Knight KR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (Drug Combinations)
RN  - 0 (Laminin)
RN  - 0 (Polymers)
RN  - 0 (Proteoglycans)
RN  - 119978-18-6 (matrigel)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Blood Vessels/*growth & development
MH  - Collagen
MH  - Drug Combinations
MH  - Extracellular Matrix/*physiology
MH  - Immunohistochemistry
MH  - Lactic Acid
MH  - Laminin
MH  - Male
MH  - Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers
MH  - Proteoglycans
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Tissue Engineering
EDAT- 2002/01/19 10:00
MHDA- 2002/02/02 10:01
CRDT- 2002/01/19 10:00
PHST- 2002/01/19 10:00 [pubmed]
PHST- 2002/02/02 10:01 [medline]
PHST- 2002/01/19 10:00 [entrez]
AID - 10.1111/j.1749-6632.2001.tb03853.x [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2001 Nov;944:429-42. doi: 10.1111/j.1749-6632.2001.tb03853.x.