PMID- 11798177
OWN - NLM
STAT- MEDLINE
DCOM- 20020219
LR  - 20141120
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 290
IP  - 3
DP  - 2002 Jan 25
TI  - Identification and immunocytochemical analysis of DCNP1, a dendritic 
      cell-associated nuclear protein.
PG  - 1022-9
AB  - Dendritic cells (DCs) are potent antigen-presenting cells (APCs). Among so-called 
      professional APCs, only DCs can activate naive T cells to initiate immune 
      response. To better understand molecular mechanisms underlying unique functions 
      of DCs, we searched for genes specifically expressed in human DCs, using 
      PCR-based cDNA subtraction in conjunction with differential screening. cDNAs 
      generated from CD34(+) stem cell-derived CD1a(+) DC were subtracted with cDNA 
      from monocytes and used for generation of a cDNA library. The cDNA library was 
      differentially screened to select genes expressed in DCs more abundantly than in 
      monocytes. We identified a gene encoding a protein composed of 244 amino acids, 
      which we designated as DCNP1 (dendritic cell nuclear protein 1). In Northern blot 
      analysis, DCNP1 mRNA was highly expressed in mature DCs and at a lower level in 
      immature DCs. In contrast, monocytes and B cells do not express the gene. In 
      multiple human tissue Northern blot analysis, expression of DCNP1 was detected in 
      brain and skeletal muscle. To examine subcellular localization of DCNP1, we 
      performed immunofluorescence analysis using an anti-DCNP1 polyclonal antibody and 
      found the molecule to be localized mainly in the perinucleus. In an 
      immunohistochemical analysis, we compared the expression of DCNP1 with CD68, a 
      marker for DCs and macrophages, in spleen, lymph node, liver, and brain. While 
      DCNP1-positive cells showed a similar tissue distribution to CD68-positive cells, 
      the number of DCNP1-positive cells was much smaller than that of CD68-positive 
      cells. Our findings are consistent with the proposal that DCNP1 is specifically 
      expressed in DCs.
FAU - Masuda, Masako
AU  - Masuda M
AD  - Division of Immunogenetics, Kumamoto University Graduate School of Medical 
      Sciences, Kumamoto, 860-0811, Japan.
FAU - Senju, Satoru
AU  - Senju S
FAU - Fujii Si, Shin-ichiro
AU  - Fujii Si S
FAU - Terasaki, Yasuhiro
AU  - Terasaki Y
FAU - Takeya, Motohiro
AU  - Takeya M
FAU - Hashimoto Si, Shin-ichi
AU  - Hashimoto Si S
FAU - Matsushima, Kouji
AU  - Matsushima K
FAU - Yumoto, Eiji
AU  - Yumoto E
FAU - Nishimura, Yasuharu
AU  - Nishimura Y
LA  - eng
SI  - GENBANK/AB074498
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (DCANP1 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, CD/analysis
MH  - Antigens, CD34/analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Dendritic Cells/chemistry/*immunology
MH  - Gene Library
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Monocytes/chemistry
MH  - Nuclear Proteins/analysis/*genetics/immunology
MH  - RNA, Messenger/biosynthesis
MH  - Tissue Distribution
EDAT- 2002/01/19 10:00
MHDA- 2002/02/20 10:01
CRDT- 2002/01/19 10:00
PHST- 2002/01/19 10:00 [pubmed]
PHST- 2002/02/20 10:01 [medline]
PHST- 2002/01/19 10:00 [entrez]
AID - S0006291X01962023 [pii]
AID - 10.1006/bbrc.2001.6202 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2002 Jan 25;290(3):1022-9. doi: 
      10.1006/bbrc.2001.6202.