PMID- 11798467
OWN - NLM
STAT- MEDLINE
DCOM- 20020328
LR  - 20081121
IS  - 1079-9907 (Print)
IS  - 1079-9907 (Linking)
VI  - 21
IP  - 12
DP  - 2001 Dec
TI  - Dysregulated response to mycobacterial cord factor trehalose-6,6'-dimycolate in 
      CD1D-/- mice.
PG  - 1089-96
AB  - The biologic effects of the mycobacterial glycolipid trehalose-6,6'-dimycolate 
      (TDM) include granuloma formation and macrophage activation and are dependent on 
      physical conformation. In mice, the group II CD1 surface molecule CD1d has been 
      implicated in glycolipid presentation. The importance of CD1d interactions in 
      pathology has yet to be established. We hypothesized that mice lacking CD1d 
      (CD1D(-/-)) would demonstrate dysregulated granulomatous response to TDM, 
      compared with CD1D(+/-) heterozygous controls. Mice were intravenously injected 
      with TDM-coated polystyrene-divinylbenzene beads and examined for histologic 
      response and for changes in inflammatory cytokine and chemokine mRNA. Control 
      CD1D heterozygous mice demonstrated a granulomatous response, which peaked at day 
      5. Increased mRNA for tumor necrosis factor-alpha (TNF-alpha) and macrophage 
      inflammatory protein-1alpha (MIP-1alpha) correlated with development of 
      granulomas, with very little change in interleukin-1beta (IL-1beta) and monocyte 
      chemoattractant protein-1 (MCP-1). In contrast, the CD1D(-/-) mice revealed 
      markedly different responses. Five days after administration, severe pulmonary 
      hemorrhage was induced. The relative size of inflammation surrounding coated bead 
      in the CD1D(-/-) mice was nearly double that induced in the CD1D(+/-) mice. 
      CD1D(-/-) mice also demonstrated elevated mRNA for both inflammatory cytokines 
      and chemokines by day 1 after administration, significantly earlier than 
      responses seen in the heterozygous controls.
FAU - Actor, J K
AU  - Actor JK
AD  - Department of Pathology, University of Texas Houston Medical School, Houston, TX 
      77030, USA. Jeffrey.K.Actor@uth.tmc.edu
FAU - Olsen, M
AU  - Olsen M
FAU - Hunter, R L Jr
AU  - Hunter RL Jr
FAU - Geng, Y J
AU  - Geng YJ
LA  - eng
GR  - R01-HL55969-01/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Antigens, CD1)
RN  - 0 (Antigens, CD1d)
RN  - 0 (Chemokines)
RN  - 0 (Cord Factors)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Antigens, CD1/*genetics
MH  - Antigens, CD1d
MH  - Chemokines/biosynthesis/genetics
MH  - Cord Factors/*pharmacology
MH  - Cytokines/biosynthesis/genetics
MH  - Granuloma, Respiratory Tract/immunology/*microbiology/pathology
MH  - Hemorrhage/microbiology/pathology
MH  - Lung Diseases/immunology/*microbiology/pathology
MH  - Mice
MH  - Mice, Knockout
MH  - Mycobacterium/*pathogenicity
MH  - Pneumonia/immunology/microbiology
MH  - RNA, Messenger/biosynthesis
EDAT- 2002/01/19 10:00
MHDA- 2002/03/29 10:01
CRDT- 2002/01/19 10:00
PHST- 2002/01/19 10:00 [pubmed]
PHST- 2002/03/29 10:01 [medline]
PHST- 2002/01/19 10:00 [entrez]
AID - 10.1089/107999001317205222 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2001 Dec;21(12):1089-96. doi: 
      10.1089/107999001317205222.