PMID- 11798663
OWN - NLM
STAT- MEDLINE
DCOM- 20060124
LR  - 20140226
IS  - 0578-1426 (Print)
IS  - 0578-1426 (Linking)
VI  - 38
IP  - 5
DP  - 1999 May
TI  - [Effects of Kupffer cells stimulated by triglyceride and very low-density 
      lipoprotein on proliferation of rat hepatic stellate cells].
PG  - 313-5
AB  - OBJECTIVE: To study the effects of Kupffer cell-conditioned medium (KCCM) derived 
      from triglyceride and very low-density lipoprotein (VLDL) treatment on 
      proliferation of rat hepatic stellate cells (HSC). METHODS: HSC and Kupffer cells 
      were isolated from liver of Wistar rats by in situ perfusion with pronase and 
      collagenase and density gradient centrifugation with Nycodenz and then cultured. 
      KCCM was prepared and MTT colorimetric assay was used to detect HSC 
      proliferation. RESULTS: HSC and Kupffer cells were isolated successfully with 
      high purity. 12.5 mg/L of triglyceride and 25 - 100 mg/L of VLDL promoted HSC 
      proliferation (P < 0.05 or 0.01); KCCM + triglyceride and KCCM + VLDL might 
      promote HSC proliferation as compared with a control group (P < 0.01). There was 
      no significant difference between KCCM + triglyceride and KCCM + VLDL (P > 0.05). 
      CONCLUSION: The technique for isolation of HSC and Kupffer cells described here 
      is simple and reliable. Triglyceride, VLDL and KCCM stimulated by them may 
      promote HSC proliferation and be associated with fatty liver and hepatic 
      fibrogenesis.
FAU - Lu, L
AU  - Lu L
AD  - Shanghai Institute of Digestive Diseases, Renji Hospital, Shanghai Second Medical 
      University, Shanghai 200001.
FAU - Zeng, M
AU  - Zeng M
FAU - Li, J
AU  - Li J
FAU - Hua, J
AU  - Hua J
FAU - Fan, J
AU  - Fan J
FAU - Fan, Z
AU  - Fan Z
FAU - Dai, N
AU  - Dai N
FAU - Qiu, D
AU  - Qiu D
FAU - Xiao, S
AU  - Xiao S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Nei Ke Za Zhi
JT  - Zhonghua nei ke za zhi
JID - 16210490R
RN  - 0 (Lipoproteins, VLDL)
RN  - 0 (Triglycerides)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Hepatocytes/*cytology
MH  - Kupffer Cells/cytology/*drug effects
MH  - Lipoproteins, VLDL/*pharmacology
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Triglycerides/*pharmacology
EDAT- 2002/01/19 10:00
MHDA- 2006/01/25 09:00
CRDT- 2002/01/19 10:00
PHST- 2002/01/19 10:00 [pubmed]
PHST- 2006/01/25 09:00 [medline]
PHST- 2002/01/19 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Nei Ke Za Zhi. 1999 May;38(5):313-5.