PMID- 11798889
OWN - NLM
STAT- MEDLINE
DCOM- 20020419
LR  - 20131121
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 81
IP  - 5
DP  - 2001 Mar 10
TI  - [Relation between BDNF and synaptic reorganization of hippocampal mossy fibers].
PG  - 283-7
AB  - OBJECTIVE: To investigate the relation between brain-derived neurotrophic factor 
      (BDNF) and synaptic reorganization of hippocampal fibers (MF). METHODS: Antisense 
      or sense oligomucleotides to BDNF and/or PBS buffer were injected into the 
      cerebal ventricles of rats of epileptic model induced by kainic acid (KA) and 
      control rats respectively. Immunohistochemical staining and Timm's silver sulfide 
      staining were used to examine the effect of antisense oligonucleotides to BDNF 
      upon the MF sprouting in the KA model and control rats. RESULTS: Timm's staining 
      showed that multiple aberrant Timm's granules were found in the inner molecular 
      layer (IML) of the dentate gyrus of hippocampus of the KA model rats 
      intracerebroventricularly injected with sense oligonucleotides to BDNF or PBS, 
      less aberrant Timm's granules were found in the hippocampal IML of KA model rats 
      intracerebroventricularly injected with antisense oligonucleotides to BDNF, and 
      no aberrant Timm's granule was found in the hippocampal IML of non-KA model rats 
      intracerebroventricular injected with antisense oligonucleotides to BDNF. 
      Immunohistochemical staining showed multiple BDNF immunologically labeled neurons 
      in the hilus and CA3 region of hippocampus of KA model rats 
      intracerebroventricularly injected with sense oligonucleotides to BDNF and PBS, 
      less in the KA model rats intracerebroventricularly injected with antisense 
      oligonucleotides to BDNF, and only a few in the non-KA model rats. The A value in 
      both Timm's and immunohistochemical sections of KA model rats was higher than 
      that of non-KA model rats. The A value in both Timm's and immunohistochemical 
      sections of KA model rats was significantly lower in the groups injected with 
      antisense oligonucleotides to BDNF than in the group injected with sense 
      oligonucleotides. CONCLUSION: BDNF may play an important role in promoting the MF 
      sprouting caused by repeated epileptic convulsion in the course of MF synaptic 
      reorganization. The expression of BDNF protein can be upregulated by subcutaneous 
      injection of KA and be decreased by intracerebroventricular injection of 
      antisense oligonucleotides to BDNF.
FAU - Zhao, S
AU  - Zhao S
AD  - Division of Neurology, Department of Pediatrics, First Hospital Affiliated to 
      Peking University, Beijing 100034, China.
FAU - Jiang, Y
AU  - Jiang Y
FAU - Luo, Q
AU  - Luo Q
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA, Antisense)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/analysis/genetics/*physiology
MH  - Chromosome Pairing/drug effects/*physiology
MH  - DNA, Antisense/pharmacology
MH  - Epilepsy/chemically induced/metabolism/physiopathology
MH  - Hippocampus/chemistry/metabolism/physiopathology
MH  - Immunohistochemistry
MH  - Injections, Intraventricular
MH  - Kainic Acid/administration & dosage
MH  - Mossy Fibers, Hippocampal/drug effects/*metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2002/01/19 10:00
MHDA- 2002/04/20 10:01
CRDT- 2002/01/19 10:00
PHST- 2002/01/19 10:00 [pubmed]
PHST- 2002/04/20 10:01 [medline]
PHST- 2002/01/19 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2001 Mar 10;81(5):283-7.