PMID- 11803122
OWN - NLM
STAT- MEDLINE
DCOM- 20020321
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 318
IP  - 3
DP  - 2002 Feb 1
TI  - Turnover analysis of N-methyl-D-aspartate receptor subunit NR1 protein in PC12 
      cells.
PG  - 153-7
AB  - The post-translational fate of N-methyl-D-aspartate receptor (NMDAR) subunit NR1 
      was characterized in PC12 cells using pulse-chase labeling, block of protein 
      synthesis by cyclohexamide and deglycosylation by endoglycosidase H. Metabolic 
      labeling of NR1 protein indicated a biphasic degradation of NR1 protein with 
      half-lives of 1.6 and 16.1 h for a rapidly (78%) and a slowly (22%) degrading 
      population. Immunoprecipitation of NR1 following the block of protein synthesis 
      by cyclohexamide revealed that the rapidly and slowly degrading pools mainly 
      consisted of the NR1 splice variants NR1-4a and NR1-2a. Sensitivity of NR1 
      protein to deglycosylation by endoglycosidase H indicated the presence of an 
      immature form of NR1 that was retained in the endoplasmic reticulum. PC12 cells 
      serve as a useful model for the elucidation of translational and 
      post-translational mechanisms of NMDAR expression.
FAU - Vazhappilly, Rema
AU  - Vazhappilly R
AD  - Biotechnology Research Institute and Department of Biology, Hong Kong University 
      of Science and Technology, Clear Water Bay, Kowloon, SAR, Hong Kong, China.
FAU - Sucher, Nikolaus J
AU  - Sucher NJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (NR1 NMDA receptor)
RN  - 0 (Protein Isoforms)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 98600C0908 (Cycloheximide)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
SB  - IM
MH  - Alternative Splicing/*physiology
MH  - Animals
MH  - Calcium Signaling/drug effects/physiology
MH  - Central Nervous System/*metabolism
MH  - Cycloheximide/pharmacology
MH  - Endoplasmic Reticulum/metabolism
MH  - Gene Expression Regulation/physiology
MH  - Glutamic Acid/*metabolism
MH  - Glycoside Hydrolases/metabolism/pharmacology
MH  - Immunohistochemistry
MH  - Neurons/*metabolism
MH  - PC12 Cells
MH  - Protein Biosynthesis/physiology
MH  - Protein Isoforms/genetics/metabolism
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Receptors, N-Methyl-D-Aspartate/*genetics/*metabolism
MH  - Synaptic Transmission/*physiology
EDAT- 2002/01/23 10:00
MHDA- 2002/03/22 10:01
CRDT- 2002/01/23 10:00
PHST- 2002/01/23 10:00 [pubmed]
PHST- 2002/03/22 10:01 [medline]
PHST- 2002/01/23 10:00 [entrez]
AID - S030439400102482X [pii]
AID - 10.1016/s0304-3940(01)02482-x [doi]
PST - ppublish
SO  - Neurosci Lett. 2002 Feb 1;318(3):153-7. doi: 10.1016/s0304-3940(01)02482-x.